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An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels.
TLDR
3,3′,5,5′-tetramethylbenzidine-H2O2 as a stain for the peroxidase activity of cytochrome P-450 in sodium dodecyl sulfate polyacrylamide gels can be used to detect very low levels of heme-associated peroxIDase activity. Expand
Characterization of the oxidative metabolites of 17beta-estradiol and estrone formed by 15 selectively expressed human cytochrome p450 isoforms.
TLDR
Many human CYP isoforms are involved in the oxidative metabolism of 17beta-estradiol and estrone, with a varying degree of catalytic activity and distinct regioselectivity. Expand
MAPK Pathways Activate and Phosphorylate the Osteoblast-specific Transcription Factor, Cbfa1*
TLDR
This study is the first demonstration that Cbfa1 is controlled by MAPKs and suggests that this pathway has an important role in the control of osteoblast-specific gene expression. Expand
Inhibition and kinetics of cytochrome P4503A activity in microsomes from rat, human, and cdna-expressed human cytochrome P450.
TLDR
Inhibition studies showed that MDZ hydroxylation was strongly inhibited by ketoconazole and Ro 23-7637 in rat, human, and cDNA-expressed human CYP3A4 microsomes, thus suggesting that members of the CYP 3A4 subfamily were involved in the metabolism. Expand
Cytochrome P450 enzymes involved in acetaminophen activation by rat and human liver microsomes and their kinetics.
TLDR
Using this approach, involvement of enzymes in the activation of APAP and their kinetics were studied and substantial activity was observed in rat liver microsomes and human P450s expressed in Hep G2 cells. Expand
Role of NF-kappaB in regulation of PXR-mediated gene expression: a mechanism for the suppression of cytochrome P-450 3A4 by proinflammatory agents.
TLDR
It is reported that NF-kappaB activation by lipopolysaccharide and tumor necrosis factor-alpha plays a pivotal role in the suppression of cyp3a4 through interactions of NF-KappaB with the PXR, and NF- kappaB p65 directly interacted with the DNA-binding domain of RXRalpha and may prevent its binding to the consensus DNA sequences, thus inhibiting the transactivation by the PxR. Expand
Methoxyresorufin and benzyloxyresorufin: substrates preferentially metabolized by cytochromes P4501A2 and 2B, respectively, in the rat and mouse.
TLDR
It would appear that MTR is a relatively specific substrate for CYP1A2 activity in rodents, while BZR appears to be relatively specific for CyP2B. Expand
PCBs: structure–function relationships and mechanism of action
TLDR
There was an excellent correlation between AHH induction potencies and receptor binding avidities of these compounds and the order of activity was coplanar PCBs, the halogenated aromatic hydrocarbons which exhibit the highest binding affinities for the receptor protein. Expand
Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats.
TLDR
The studies show that constitutive expression and induction of steroid-inducible cytochrome P450s may vary as a function of age. Expand
Effects of phenethyl isothiocyanate, a carcinogenesis inhibitor, on xenobiotic-metabolizing enzymes and nitrosamine metabolism in rats.
TLDR
The study demonstrates that PEITC selectively affects xenobiotic-metabolizing enzymes in the liver, lung and nasal mucosa and it is especially effective in inhibiting the P450-dependent oxidation of NNK in the lung and of NDMA in the Liver. Expand
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