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Universal primers for amplification of three non-coding regions of chloroplast DNA
TLDR
Six primers for the amplification of three non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed and worked for most species tested, which means that they may be used to study the population biology and evolution of plants. Expand
Estimating the probability of identity among genotypes in natural populations: cautions and guidelines
TLDR
An equation for P(ID) between sibs is introduced that provides an estimator that can be used as a conservative upper bound for the probability of observing identical multilocus genotypes between two individuals sampled from a population. Expand
Comparative phylogeography and postglacial colonization routes in Europe
TLDR
A Brooks parsimony analysis produced an unrooted area phylogram, showing that: (i) the northern regions were colonized generally from the Iberic and Balkanic refugia; and (ii) the Italian lineages were often isolated due to the presence of the Alpine barrier. Expand
How to track and assess genotyping errors in population genetics studies
TLDR
Four case studies representing a large variety of population genetics investigations differing in their sampling strategies, in the type of organism studied (plant or animal) and the molecular markers used [microsatellites or amplified fragment length polymorphisms (AFLPs), and the estimated genotyping error rate are considered. Expand
Landscape genetics: combining landscape ecology and population genetics
TLDR
A new approach has emerged for analyzing spatial genetic data without requiring that discrete populations be identified in advance, and promises to facilitate the understanding of how geographical and environmental features structure genetic variation at both the population and individual levels. Expand
Genotyping errors: causes, consequences and solutions
TLDR
A protocol for estimating error rates is proposed and it is recommended that these measures be systemically reported to attest the reliability of published genotyping studies. Expand
Reliable genotyping of samples with very low DNA quantities using PCR.
TLDR
An experimental procedure using PCR that provides a reliable genotype at a microsatellite locus using only a few picograms of template DNA is identified and should be systematically used when genotyping nuclear loci of ancient or forensic samples, museum specimens and hair or feces of free ranging animals. Expand
Species detection using environmental DNA from water samples
TLDR
A novel approach, based on the limited persistence of DNA in the environment, to detect the presence of a species in fresh water, using specific primers that amplify short mitochondrial DNA sequences to track the existence of a frog in controlled environments and natural wetlands. Expand
Who is eating what: diet assessment using next generation sequencing
TLDR
The power and pitfalls of NGS diet methods are reviewed, the critical factors to take into account when choosing or designing a suitable barcode are presented and the validation of data accuracy including the viability of producing quantitative data is discussed. Expand
A spatial analysis method (SAM) to detect candidate loci for selection: towards a landscape genomics approach to adaptation
TLDR
A new method to detect signatures of natural selection based on the application of spatial analysis, with the contribution of geographical information systems (GIS), environmental variables and molecular data is introduced. Expand
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