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Mechanism of eukaryotic homologous recombination.
HR accessory factors that facilitate other stages of the Rad51- and Dmc1-catalyzed homologous DNA pairing and strand exchange reaction have also been identified.
Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase.
- P. Sung
- BiologyGenes & development
- 1 May 1997
By several independent criteria, RAD55- and RAD57-encoded products are shown here to exist as a stable heterodimer, providing evidence for a role of the Rad55-Rad57heterodimer in overcoming the inhibitory effect of RPA.
Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p.
Function of Yeast Rad52 Protein as a Mediator between Replication Protein A and the Rad51 Recombinase*
- P. Sung
- Biology, ChemistryThe Journal of Biological Chemistry
- 7 November 1997
Rad52 functions as a co-factor for the Rad51 recombinase, acting specifically to overcome the apparent competition by RPA for binding to single-stranded DNA.
DNA helicase Srs2 disrupts the Rad51 presynaptic filament
The role of SRS2 in recombination modulation is clarified by purifying its encoded product and examining its interactions with the Rad51 recombinase, and it is shown that Srs2 acts by dislodging Rad51 from ssDNA.
Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae
It is shown that DNA strand separation during end resection is mediated by the Sgs1 helicase function, in a manner that is enhanced by Top3–Rmi1 and MRX, and a multifaceted role of RPA is unveiled, in the sequestration of ssDNA generated by DNA unwinding, enhancement of 5′ strand incision, and protection of the 3′ strand.
Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein.
- P. Sung
- Biology, ChemistryScience
- 26 August 1994
The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair and it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from phi X 174 or M13 with its respective homologous linear double-Stranded form, indicating that RAD 51 can catalyze strand exchange.
Mechanism of homologous recombination: mediators and helicases take on regulatory functions
This work has shown that mutations in the tumour-suppressor protein BRCA2, which has a mediator function in HR, lead to cancer formation and DNA helicases, such as Bloom's syndrome protein, regulate HR at several levels, in attenuating unwanted HR events and in determining the outcome of HR.
RECQL5/Recql5 helicase regulates homologous recombination and suppresses tumor formation via disruption of Rad51 presynaptic filaments.
This work shows that deletion of Recql5 in mice results in cancer susceptibility and identifies RECQL5 as an important tumor suppressor that may act by preventing inappropriate HR events via Rad51 presynaptic filament disruption.
Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins
It is concluded that Rad54 acts to overcome kinetic impediments that would limit homologous DNA pairing between recombining chromosomes in vivo.