Elevated globotriaosylsphingosine is a hallmark of Fabry disease
- J. Aerts, J. Groener, B. Poorthuis
- BiologyProceedings of the National Academy of Sciences
- 26 February 2008
It is shown that globotriaosylsphingosine is an inhibitor of α-galactosidase A activity and the increased intima-media thickness in Fabry patients therefore may be related to the presence of this metabolite.
Phosphatidylserine is polarized and required for proper Cdc42 localization and for development of cell polarity
- G. Fairn, M. Hermansson, P. Somerharju, S. Grinstein
- Biology, ChemistryNature Cell Biology
- 1 December 2011
The results indicate that phosphatidylserine—and presumably its polarization—are required for optimal Cdc42 targeting and activation during cell division and mating.
Sphingomyelin synthase-related protein SMSr controls ceramide homeostasis in the ER
- A. M. Vacaru, F. Tafesse, J. Holthuis
- Biology, ChemistryJournal of Cell Biology
- 15 June 2009
This study reports the identification of an SMS1-related (SMSr) enzyme, which catalyses the synthesis of the SM analogue ceramide phosphoethanolamine (CPE) in the ER lumen, and establishes SMSr as a key regulator of ceramide homeostasis that seems to operate as a sensor rather than a converter of ceramides in theER.
Both Sphingomyelin Synthases SMS1 and SMS2 Are Required for Sphingomyelin Homeostasis and Growth in Human HeLa Cells*
- F. Tafesse, Klazien Huitema, J. Holthuis
- BiologyJournal of Biological Chemistry
- 15 June 2007
It is shown that SMS1 and SMS2 are co-expressed in a variety of cell types and function as the key Golgi- and plasma membrane-associated SM synthases in human cervical carcinoma HeLa cells, respectively, indicating that the biological role of SM synthase goes beyond formation of SM.
Software tools for analysis of mass spectrometric lipidome data.
- P. Haimi, A. Uphoff, M. Hermansson, P. Somerharju
- PhysicsAnalytical Chemistry
- 17 November 2006
New software tools for quantitative analysis of mass spectrometric lipidome data make accurate, high-throughput analysis of extensive sample sets feasible and are made available to the scientific community free of charge.
Mechanisms of glycerophospholipid homeostasis in mammalian cells.
- M. Hermansson, K. Hokynar, P. Somerharju
- BiologyProgress in lipid research
- 1 July 2011
Dissecting the role of the golgi complex and lipid rafts in biosynthetic transport of cholesterol to the cell surface.
- S. Heino, S. Lusa, P. Somerharju, C. Ehnholm, V. Olkkonen, E. Ikonen
- BiologyProceedings of the National Academy of Sciences…
- 18 July 2000
The results provide evidence for the partial contribution of the Golgi complex to the transport of newly synthesized cholesterol to the cell surface and suggest that detergent-resistant membranes are involved in the process.
Membrane Binding Mechanism of an RNA Virus-capping Enzyme*
- A. Lampio, I. Kilpeläinen, L. Kääriäinen
- Biology, ChemistryJournal of Biological Chemistry
- 1 December 2000
Mutation W259A altered the mode of bilayer association of the peptide and abolished its ability to compete for membrane association of intact NSp1, demonstrating its crucial role in the membrane association and function of Nsp1.
Phosphatidic acid is required for the constitutive ruffling and macropinocytosis of phagocytes
- M. Bohdanowicz, Daniel Schlam, S. Grinstein
- BiologyMolecular Biology of the Cell
- 1 June 2013
Phagocytes spontaneously ruffle as they probe their environment and take up antigens. These cells are uniquely enriched in phosphatidic acid, which is necessary for ruffling and macropinocytosis.
Automated quantitative analysis of complex lipidomes by liquid chromatography/mass spectrometry.
- M. Hermansson, A. Uphoff, R. Käkelä, P. Somerharju
- Biology, ChemistryAnalytical Chemistry
- 22 February 2005
This work has developed an automated method that allows unattended identification and quantification of lipid molecular species of all the major lipid classes from a two-dimensional chromatographic/mass spectrometric data set and makes high-throughput lipidomics feasible in biology, biotechnology, and medicine.