Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction
- C. Picard, C. Ponsonnet, E. Paget, X. Nesme, P. Simonet
- Medicine, BiologyApplied and Environmental Microbiology
- 1 September 1992
A rapid and specific detection test for bacteria in soil is developed based on the polymerase chain reaction (PCR), and the strong correlation the authors observed between the size of the inocula and the results of the PCR reactions permitted assessment of the validity of the protocol in enumerating the number of microbial cells present in a soil sample.
Quantification of Bias Related to the Extraction of DNA Directly from Soils
- Åsa Frostegård, S. Courtois, P. Simonet
- Biology, MedicineApplied and Environmental Microbiology
- 1 December 1999
The aim of the present investigation was to improve the extraction, purification, and quantification of DNA derived from as large a portion of the soil microbial community as possible, with special emphasis placed on obtaining DNA from gram-positive bacteria, which form structures that are difficult to disrupt.
Recombinant Environmental Libraries Provide Access to Microbial Diversity for Drug Discovery from Natural Products
The data reinforce the idea that exploiting previously unknown or uncultivated microorganisms for the discovery of novel natural products has potential value and suggest a strategy for developing this technology into a realistic and effective drug discovery tool.
Extraction of DNA from soil
Accessing the Soil Metagenome for Studies of Microbial Diversity
This work was able to access a more genetically diverse proportion of the metagenome (a gain of more than 80% compared to the best single extraction method), limit the predominance of a few genomes, and increase the species richness per sequencing effort.
Antibiotic-resistant soil bacteria in transgenic plant fields
- S. Demanèche, H. Sanguin, P. Simonet
- Biology, MedicineProceedings of the National Academy of Sciences
- 11 March 2008
The results indicate that soil bacteria are naturally resistant to a broad spectrum of beta-lactam antibiotics, including the third cephalosporin generation, which has a slightly stronger discriminating effect on soil isolates than other cep Halosporins.
Adsorption of DNA on clay minerals: protection against DNaseI and influence on gene transfer
Clay-adsorbed DNA was found to be still able, even after a strong DNaseI treatment, to artificially transform competent Escherichia coli cells, showing that persistance of DNA and gene transfer by genetic transformation may occur in soil.
The soil resistome: a critical review on antibiotic resistance origins, ecology and dissemination potential in telluric bacteria.
Different ecological hypotheses explaining their high prevalence in soil and questioning their transfer rate to pathogens, in respect to these recent experimental results, will be discussed in the present review.
Rhodanobacter lindaniclasticus gen. nov., sp. nov., a lindane-degrading bacterium.
- R. Nalin, P. Simonet, T. Vogel, P. Normand
- BiologyInternational Journal of Systematic Bacteriology
A new genus, Rhodanobacter, containing a single species, RhodAnobacter lindaniclasticus, is proposed for strain RP5557T (= LMG 18385T), which becomes the type strain.
In Situ Transfer of Antibiotic Resistance Genes from Transgenic (Transplastomic) Tobacco Plants to Bacteria
- E. Kay, T. Vogel, Frank Bertolla, R. Nalin, P. Simonet
- Biology, EngineeringApplied and Environmental Microbiology
- 1 July 2002
It is suggested that by selecting plant transgene sequences that are nonhomologous to bacterial sequences, plant biotechnologists could restore the genetic barrier to transgenes transfer to bacteria.