P. Shaffer

Publications11
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Citations160
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Studies pertaining to the purification and properties of thymine 7-hydroxylase.
Abstract 1. 1. It has been shown that the thymine 7-hydroxylase activity of cell-free extracts prepared from cultures of Neurospora crassa, grown under forced aeration in liquid media, is usually… Expand
  • 18
Metabolism of pyrimidine deoxyribonucleosides in Neurospora crassa.
The experiments in this report involve the following series of reactions which were previously demonstrated with purified enzyme preparations from Neurospora crassa: thymidine a yields thymine… Expand
  • 17
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Regulation of thymidine metabolism in Neurospora crassa.
The utilization of thymidine by Neurospora crassa is initiated by the pyrimidine deoxyribonucleoside 2'-hydroxylase reaction and the consequent formation of thymine and ribose. Thymine must then be… Expand
  • 12
The cell-free conversion of a deoxyribonucleoside to a ribonucleoside without detachment of the deoxyribose.
Abstract A cell-free preparation has been obtained from Neurospora crassa which catalyzes the conversion of thymidine to thymine riboside. This oxidation occurs at the deoxyribonucleoside level and… Expand
  • 21
Stoichiometry of the pyrimidine deoxyribonucleoside 2'-hydroxylase reaction and of the conversions of 5-hydroxymethyluracil to 5-formyluracil and of the latter to uracil-5-carboxylic acid.
  • 18
Substrate specificity of the hydroxylase reaction in which thymidine is converted to thymine ribonucleoside.
Abstract 1. 1.|A partially purified enzyme preparation from Neurospora crassa, which had previously been reported to convert thymidine to thymine ribonucleoside, catalyzes the conversion of… Expand
  • 9
C‐terminal truncation of the transcriptional activator encoded by area in Aspergillus nidulans results in both loss‐of‐function and gain‐of‐function phenotypes
Mutations truncating as many as 143 C-terminal residues from the transcriptional activator encoded by the areA gene, mediating nitrogen metabolite repression in Aspergillus nidulans, do not… Expand
  • 24
Separation and characterization of pyrimidine deoxyribonucleoside 2'-hydroxylase and thymine 7-hydroxylase from Aspergillus nidulans.
Partially purified preparations from Aspergillus nidulans were shown to catalyze two alpha-ketoglutarate dependent dioxygenase reactions: the pyrimidine deoxyribonucleoside 2'-hydroxylase (EC… Expand
  • 6
Regulation of pyrimidine salvage in Aspergillus nidulans: A role for the major regulatory gene areA mediating nitrogen metabolite repression
SummaryThe synthesis of thymine 7-hydroxylase, an α-ketoglutarate dependent dioxygenase, is subject both to nitrogen metabolite repression and to oxygen repression, while synthesis of the other… Expand
  • 10
An asparaginase of Aspergillus nidulans is subject to oxygen repression in addition to nitrogen metabolite repression
SummaryOf five amidohydrolase activities subject to nitrogen metabolite repression in Aspergillus nidulans, l-asparaginase shows clearest evidence of also being subject to repression by atmospheric… Expand
  • 22
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