• Publications
  • Influence
Preparation of isolated rat liver cells.
  • P. Seglen
  • Biology, Medicine
  • Methods in cell biology
  • 1976
TLDR
This chapter discusses preparation of isolated rat liver cells by incubation of rat liver minces with pronase, which results in most of the liver parenchyma is digested, while nonparenchymal cells remain intact and can be recovered from the incubate. Expand
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
TLDR
A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented. Expand
Why the impact factor of journals should not be used for evaluating research
TLDR
Alternative methods for evaluating research are being sought, such as citation rates and journal impact factors, which seem to be quantitative and objective indicators directly related to published science. Expand
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
TLDR
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms. Expand
Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway.
TLDR
Genetic interaction studies showed that ecdysone signaling downregulates PI3K signaling and that this represents the effector mechanism for induction of programmed autophagy, and these studies link hormonal induction ofAutophagy to the regulatory function of the PI3k signaling pathway in vivo. Expand
3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes.
TLDR
The ability of 3-methyladenine to suppress the formation of electron microscopically visible autophagosomes suggests that it may be regarded as a specific inhibitor of autophagy. Expand
Methods for Monitoring Autophagy from Yeast to Human
TLDR
Current approaches that can be used to follow the overall process of autophagy, as well as individual steps, from yeast to human are discussed. Expand
The Skewness of Science
  • P. Seglen
  • Computer Science
  • J. Am. Soc. Inf. Sci.
  • 1992
TLDR
The citational variability between articles in a journal is less (semilog linearity) than in the corresponding field as a whole, suggesting that each journal represents a select, stratified sample of the field. Expand
Citations and journal impact factors: questionable indicators of research quality
TLDR
The traditional method of evaluation is peer review, whereby research plans or scientific results are scrutinized by scientific colleagues, assumed to possess the necessary expertise, but this method is all too often performed by narrow specialists who are unable to compare different projects. Expand
Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion.
  • P. Seglen
  • Biology, Medicine
  • Experimental cell research
  • 1 December 1973
TLDR
Isolated perfused rat livers were dispersed by a two-step procedure of Ca2+ removal (probably including the removal of a Ca2-dependent adhesion factor) followed by enzymatic treatment, and cells were active in the synthesis of RNA, protein, glycogen and various metabolites, and showed sensitivity towards steroid and polypeptide hormones. Expand
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