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Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes
Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sortingExpand
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Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy.
We have investigated the pH dependence of the dynamics of conformational fluctuations of green fluorescent protein mutants EGFP (F64L/S65T) and GFP-S65T in small ensembles of molecules in solution byExpand
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Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation.
Multiphoton excitation (MPE) of fluorescent probes has become an attractive alternative in biological applications of laser scanning microscopy because many problems encountered in spectroscopicExpand
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Characterization of lipid bilayer phases by confocal microscopy and fluorescence correlation spectroscopy.
We report the application of confocal imaging and fluorescence correlation spectroscopy (FCS) to characterize chemically well-defined lipid bilayer models for biomembranes. Giant unilamellar vesiclesExpand
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Probing Lipid Mobility of Raft-exhibiting Model Membranes by Fluorescence Correlation Spectroscopy*
Confocal fluorescence microscopy and fluorescence correlation spectroscopy (FCS) have been employed to investigate the lipid spatial and dynamic organization in giant unilamellar vesicles (GUVs)Expand
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GM1 structure determines SV40-induced membrane invagination and infection
Incoming simian virus 40 (SV40) particles enter tight-fitting plasma membrane invaginations after binding to the carbohydrate moiety of GM1 gangliosides in the host cell plasma membrane throughExpand
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Spatial Regulators for Bacterial Cell Division Self-Organize into Surface Waves in Vitro
In the bacterium Escherichia coli, the Min proteins oscillate between the cell poles to select the cell center as division site. This dynamic pattern has been proposed to arise by self-organizationExpand
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Fluorescence correlation spectroscopy: novel variations of an established technique.
Fluorescence correlation spectroscopy (FCS) is one of the major biophysical techniques used for unraveling molecular interactions in vitro and in vivo. It allows minimally invasive study of dynamicExpand
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Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules
It is widely accepted that tissue differentiation and morphogenesis in multicellular organisms are regulated by tightly controlled concentration gradients of morphogens. How exactly these gradientsExpand
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Fluorescence correlation spectroscopy relates rafts in model and native membranes.
The lipid raft model has evoked a new perspective on membrane biology. Understanding the structure and dynamics of lipid domains could be a key to many crucial membrane-associated processes in cells.Expand
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