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Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid, and the sequenced genome revealed a large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors. Expand
RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus.
The structure and function of the effector complex of the Type III-A CRISPR-Cas system of Thermus thermophilus: the Csm complex is reported, showing that, like TtCmr, TTCsm cleaves complementary target RNAs at multiple sites. Expand
Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus.
Electron microscopy revealed that the structure of TtCmr resembles a "sea worm" and is composed of a Cmr2-3 heterodimer "tail," a helical backbone of Cmr4 subunits capped by Cmr5 subunits, and a curled "head" containing Cmr1 and Cmr6. Expand
An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation.
Transcriptome analysis of Aspergillus niger transfer cultures grown on galacturonic acid media identified a highly correlating cluster of four strongly induced hypothetical genes linked with a subset set of genes encoding pectin degrading enzymes, which strongly suggest that a common d-galactuonic acid utilization pathway is operative in these species. Expand
Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88.
A whole-genome sequencing of the acidogenic A. niger wild-type strain resulted in a snapshot of fungal evolution and will support further optimization of cell factories based on filamentous fungi. Expand
Assessment of the pectin degrading enzyme network of Aspergillus niger by functional genomics.
The saprobic fungus Aspergillus niger is an efficient producer of a suite of extracellular enzymes involved in carbohydrate modification and degradation and the prediction of at least 39 genes involved in the depolymerisation of the backbone of pectin is predicted. Expand
Genome-scale metabolic model for Lactococcus lactis MG1363 and its application to the analysis of flavor formation
The genome-scale metabolic model couples the carbon and nitrogen metabolism in L. lactis MG1363 with complete known catabolic pathways leading to flavor formation and has the potential to contribute to new developments in dairy industries and cheese-flavor research. Expand
A genomic view on syntrophic versus non-syntrophic lifestyle in anaerobic fatty acid degrading communities.
In sulfate-reducing and methanogenic environments complex biopolymers are hydrolyzed and degraded by fermentative micro-organisms that produce hydrogen, carbon dioxide and short chain fatty acids.Expand
Identification and functional analysis of the nuclear localization signals of ribosomal protein L25 from Saccharomyces cerevisiae.
Microinjection of in vitro prepared intact and N-terminally truncated L25 into Xenopus laevis oocytes demonstrated that the region containing the two NLS sequences is indeed required for efficient nuclear localization of the ribosomal protein. Expand