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A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
Abstract A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented. The ferric iron is… Expand
Catalytic activity of administered gulonolactone oxidase polyethylene glycol conjugates.
Scurvy in guinea pigs provides a convenient model of inborn metabolic disease for the investigation of enzyme therapy protocols. Gulonolactone oxidase, the enzyme in ascorbic acid biosynthesis that… Expand
Ascorbic acid: an endogenous inhibitor of isolated Na+,K+-ATPase.
During attempts to isolate and identify an endogenous ligand for the glycoside binding sites on Na+,K+-ATPase, bovine adrenal glands were found to contain a potent inhibitor of isolated… Expand
EFFECTS OF ASCORBIC ACID ON MICROSOMAL DRUG METABOLISM *
Studies from a number of laboratories have shown that vitamin C deficiency in guinea pigs results in decreased metabolism of a variety of pharmacological agents.'-'" In 1941, Richards et al.' found a… Expand
Ascorbic acid and hepatic drug metabolism.
Previous in vivo studies indicate that hepatic microsomal drug metabolism decreases in ascorbic acid deficiency and is augmented when high supplements of the vitamin are given to guinea pigs. Kinetic… Expand
Tirilazad Mesylate Protects Vitamins C and E in Brain Ischemia‐Reperfusion Injury
Abstract: Brain concentrations of the antioxidant vitamins C and E decreased following unilateral carotid occlusion and reperfusion for 2 or 24 h in gerbils. Administration of the 21‐aminosteroid… Expand
Glutaraldehyde-reacted immunoprecipitates of L-gulonolactone oxidase are suitable for administration to guinea pigs.
The potential pharmacologic use of enzymes has long been considered. Practical applications, however, have been limited by the toxicity and allergic response to administered foreign proteins. A… Expand
Renal L-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon (Acipenser fulvescens)
Abstract Sturgeon can synthesize l -ascorbic acid (vitamin C) as they possess in their kidney l -gulono-1,4-lactone oxidase, the enzyme catalyzing the last step of AA biosynthesis. The effect of… Expand
A protocol for the successful long-term enzyme replacement therapy of scurvy in guinea pigs
SummaryGulonolactone oxidase, a key enzyme in the biosynthesis of ascorbic acid, is missing from guinea pigs and certain other scurvy-prone species. Weekly intraperitoneal injections of… Expand
Studies on ascorbic acid related to the genetic basis of scurvy.
Publisher Summary This chapter presents the studies on ascorbic acid related to the genetic basis of scurvy. The importance of ascorbic acid has long been known, scurvy being the first recognized… Expand