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Light Sheet Fluorescence Microscopy
- P. Santi
- BiologyThe journal of histochemistry and cytochemistry…
- 1 February 2011
This review describes development of the technology, reviews existing devices, provides details of one LSFM device, and shows examples of images and three-dimensional reconstructions of tissues that were produced by LSFm.
Thin-sheet laser imaging microscopy for optical sectioning of thick tissues.
- P. Santi, Shane B. Johnson, M. Hillenbrand, Patrick Z GrandPre, Tiffany J Glass, J. Leger
- Biology, PhysicsBioTechniques
- 1 April 2009
A modular and optimized thin-sheet laser imaging microscope (TSLIM) for nondestructive optical sectioning of organisms and thick tissues such as the mouse cochlea, zebrafish brain/inner ear, and rat brain at a resolution that is comparable to wide-field fluorescence microscopy is reported.
Temporal Bone Histopathology in Alport Syndrome
Investigation of histopathologic abnormalities within the cochlea in Alport syndrome finds at least three new mechanisms that may be related to EMT, including the presence of EMT-like deposits in the EMT tract.
Conditional deletion of N‐Myc disrupts neurosensory and non‐sensory development of the ear
- B. Kopecky, P. Santi, Shane D. Johnson, Heather M. Schmitz, Bernd Fritzsch
- BiologyDevelopmental dynamics : an official publication…
- 1 June 2011
N‐Myc CKOs had reduced growth of the ear, abnormal morphology including fused sensory epithelia, disrupted histology, and disorganized neuronal innervation, and the abnormally developed cochlea had cross innervation from the vestibular ganglion near the basal tip.
Systemic Lipopolysaccharide Compromises the Blood-Labyrinth Barrier and Increases Entry of Serum Fluorescein into the Perilymph
- K. Hirose, J. Hartsock, Shane B. Johnson, P. Santi, A. Salt
- Biology, MedicineJournal of the Association for Research in…
- 21 June 2014
An in vivo method is developed to assess the functional integrity of the blood-labyrinth barrier by injecting sodium fluorescein into the systemic circulation of mice and measuring the amount of fluorscein that enters perilymph in live animals.
Structure of Outer Hair Cell Stereocilia Side and Attachment Links in the Chinchilla Cochlea
The structure and symmetry of chinchilla outer hair cell (OHC) stereocilia side and attachment links were investigated using tannic acid and Cuprolinic blue histochemical procedures and appeared as electron-dense, membrane-bound granular structures arranged with radial symmetry.
MicroCT versus sTSLIM 3D Imaging of the Mouse Cochlea
- J. Buytaert, Shane B. Johnson, M. Dierick, Wasil H. M. Salih, P. Santi
- PhysicsThe journal of histochemistry and cytochemistry…
- 28 January 2013
We made a qualitative and quantitative comparison between a state-of-the-art implementation of micro-Computed Tomography (microCT) and the scanning Thin-Sheet Laser Imaging Microscopy (sTSLIM)…
Nanocomposite scintillators for radiation detection and nuclear spectroscopy
Structure of outer hair cell stereocilia links in the chinchilla
The structure of side, tip, and “attachment” links of chinchilla outer hair cell (OHC) stereocilia was studied by transmission and scanning electron microscopy using tannic acid and Cuprolinic blue histochemical procedures and reactive structures appear to contain acidic, sulfated residues of proteoglycans or glycoproteins.