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Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma

A better understanding of mycoparasitism is offered, and the development of improved biocontrol strains for efficient and environmentally friendly protection of plants is enforced.
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UP-PCR analysis and ITS1 ribotyping of strains of Trichoderma and Gliocladium

The results demonstrate that the combination of UP-PCR and ribotyping can aid in clarifying species distinction in Trichoderma and Gliocladium and has the potential to become a valuable tool for studies of diversity and genetic structure of populations of these fungi.
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Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials

The important role beta-glucosidases play in complex biomass hydrolysis and how they create a bottleneck in industrial use of lignocellulosic materials is discussed and better alternatives to the currently available enzyme preparations are searched for.
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Production of cellulolytic enzymes from ascomycetes: comparison of solid state and submerged fermentation

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Identification of a Universally Primed-PCR-Derived Sequence-Characterized Amplified Region Marker for an Antagonistic Strain of Clonostachys rosea and Development of a Strain-Specific PCR Detection…

A PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers that are useful for monitoring biological control agents released into natural field soil are developed.
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UP-PCR genotyping and rDNA analysis of Ascochyta pisi Lib

In studying genetic relatedness by UP-PCR among A. pisi isolates, two distinct clusters were revealed and the markers were suitable in identifying isolates and may be useful for developing isolate- or pathotype-specific PCR-based diagnostic assays.
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Onsite Enzyme Production During Bioethanol Production from Biomass: Screening for Suitable Fungal Strains

IBT25747 (Aspergillus niger) and strain AP were found as promising candidates for onsite enzyme production where the filter cake was inoculated with the respective fungus and in combination with Celluclast 1.5L used for hydrolysis of pre-treated biomass.
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Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella

ABSTRACT A real-time, genus-specific 5′ nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The…
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Toward an International Standard for PCR-Based Detection of Food-Borne Thermotolerant Campylobacters: Validation in a Multicenter Collaborative Trial

The results indicate that the PCR assay to detect food-borne thermotolerant campylobacters can become an international standard and can be confidently applied in microbiological laboratories.
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Point mutation of the xylose reductase (XR) gene reduces xylitol accumulation and increases citric acid production in Aspergillus carbonarius

Enhanced production of citric acid can be achieved using xylose as the sole carbon source by reducing accumulation of other by-products, such as xylitol, in A. carbonarius.
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