• Publications
  • Influence
Glycogen and its metabolism.
  • P. Roach
  • Chemistry, Medicine
  • Current molecular medicine
  • 28 February 2002
Though the number of enzymes directly involved in the metabolism of glycogen is quite small, many more proteins act indirectly in a regulatory capacity and, with some exceptions, mutations in the regulatory proteins appear to cause a more subtle phenotypic change. Expand
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms. Expand
Glycogen and its metabolism: some new developments and old themes.
Significant new developments in eukaryotic glycogen metabolism over the last decade or so include: (i) three-dimensional structures of the biosynthetic enzymes glycogenin and glycogen synthase, with associated implications for mechanism and control; (ii) analyses of several genetically engineered mice with altered glycogens metabolism that shed light on the mechanism of control. Expand
Glycogen synthase kinase-3 beta is a dual specificity kinase differentially regulated by tyrosine and serine/threonine phosphorylation.
GSK-3 beta is a dual specificityprotein kinase in the same sense as the mitogen-activated protein kinase/ERK family of enzymes, and phosphorylation at different residues differentially controls enzyme activity. Expand
A secondary phosphorylation of CREB341 at Ser129 is required for the cAMP-mediated control of gene expression. A role for glycogen synthase kinase-3 in the control of gene expression.
It is proposed that the hierarchical phosphorylation at the PKA and GSK-3 sites of CREB are essential for cAMP control ofCREB. Expand
New Insights Into the Role and Mechanism of Glycogen Synthase Activation by Insulin
The metabolism of the storage polysaccharide glycogen is intimately linked with insulin action and blood glucose homeostasis, and a newer model, in which insulin inactivates the enzyme glycogen synthase kinase-3 via the protein kinase B pathway, has emerged, Though promising, this model still does not completely explain the molecular basis for the insulin-mediated activation of glycogen synthesis. Expand
Antagonistic Controls of Autophagy and Glycogen Accumulation by Snf1p, the Yeast Homolog of AMP-Activated Protein Kinase, and the Cyclin-Dependent Kinase Pho85p
  • Zhong Wang, Wayne A. Wilson, Marie A. Fujino, P. Roach
  • Medicine, Biology
  • Molecular and Cellular Biology
  • 1 September 2001
It is proposed that Snf1p and Pho85p are, respectively, positive and negative regulators of autophagy, probably via Apg1 and/or Apg13, and defective glycogen storage in snf1cells can be attributed to both defective synthesis upon entry into stationary phase and impaired maintenance of glycogen levels caused by the lack of Autophagy. Expand
Regulation of glycogen metabolism in yeast and bacteria.
This review aims to highlight the mechanisms, both at the transcriptional and at the post-transcriptional level, that regulate glycogen metabolism in yeast and bacteria, focusing on selected areas where the greatest increase in knowledge has occurred during the last few years. Expand
Structure and Molecular Mechanism of a Nucleobase–Cation–Symport-1 Family Transporter
The 2.85-angstrom resolution structure of the NCS1 benzyl-hydantoin transporter, Mhp1, from Microbacterium liquefaciens is reported, revealing that the outward- and inward-facing cavities are symmetrically arranged on opposite sides of the membrane. Expand