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Minimal Requirements for Exponential Growth of Lactococcus lactis
TLDR
It is shown that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.
The Serine/Threonine/Tyrosine Phosphoproteome of the Model Bacterium Bacillus subtilis*S
TLDR
A significantly enlarged number of bacterial proteins known to be phosphorylated on Ser/Thr/Tyr residues strongly supports the emerging view that protein phosphorylation is a general and fundamental regulatory process, not restricted only to eukaryotes, and opens the way for its detailed functional analysis in bacteria.
The Glycolytic Flux in Escherichia coli Is Controlled by the Demand for ATP
TLDR
A molecular genetic tool is developed that specifically induces ATP hydrolysis in living cells without interfering with other aspects of metabolism, and shows that the majority of the control of growth rate resides in the anabolic reactions, i.e., the cells are mostly "carbon" limited.
Twofold Reduction of Phosphofructokinase Activity in Lactococcus lactis Results in Strong Decreases in Growth Rate and in Glycolytic Flux
TLDR
Two mutant strains of Lactococcus lactis in which the promoter of the las operon was replaced by synthetic promoters, harboring pfk, pyk, and ldh, had an approximately twofold decrease in the activity of phosphofructokinase, whereas the activities of pyruvate kinase and lactate dehydrogenase remained closer to the wild-type level.
Carbon and energy metabolism of atp mutants of Escherichia coli
TLDR
Data are interpreted as indicating that E. coli makes use of its ability to respire even if it cannot directly couple this ability to ATP synthesis; by respiring away excess reducing equivalents E. Escherichia coli enhances substrate level ATP synthesis.
Modulation of Gene Expression Made Easy
TLDR
There is a linear correlation between the expressions of the two genes, which facilitates screening for mutants with suitable enzyme activities, and it is shown that the method can be applied to modulating the expression of native genes on the chromosome.
Experimental determination of control of glycolysis in Lactococcus lactis
TLDR
A genetic approach that is well suited for both Metabolic Optimization and Metabolic Control Analysis and studies on the importance of a number of glycolytic enzymes for metabolic fluxes in Lactococcus lactis are reviewed.
The Ser/Thr/Tyr phosphoproteome of Lactococcus lactis IL1403 reveals multiply phosphorylated proteins
TLDR
Evolutionary comparison and the conservation of phosphorylation sites in different bacterial organisms indicate that a majority of the detected phosphorylated sites are species–specific, and therefore have probably co‐evolved with the adaptation of the bacterial species to their present‐day ecological niches.
Polyamines Are Required for Virulence in Salmonella enterica Serovar Typhimurium
TLDR
Interestingly, intracellular survival of the polyamine mutant was significantly enhanced above the wild type level by the addition of exogenous putrescine and spermidine to the bacterial cultures prior to infection, indicating that these polyamines function as an environmental signal that primes S. Typhimurium for intrACEllular survival.
Bioconversion of crude glycerol feedstocks into ethanol by Pachysolen tannophilus.
TLDR
This study demonstrates a robust bioprocess which was not sensitive to the batch variability in crude glycerol dependent on raw materials used for biodiesel production, and the oxygen transfer rate (OTR) was a key factor for ethanol production.
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