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Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction.
TLDR
Sets of parameters governing the rules were determined under various mutagenic conditions including the addition of MnCl2 and validity of the rules was assessed in several mutagenesis experiments showing that a wide range of substitution frequencies including AT-->GC and GC-->AT transitions as well as AT-->TA transversions can be obtained at will. Expand
Crystal structure at 1.2 Å resolution and active site mapping of Escherichia coli peptidyl‐tRNA hydrolase
TLDR
The structure of crystalline peptidyl‐tRNA hydrolase could be solved at 1.2 Å resolution and indicates a single α/β globular domain built around a twisted mixed β‐sheet, similar to the central core of an aminopeptidase from Aeromonas proteolytica. Expand
Role of d-Cysteine Desulfhydrase in the Adaptation of Escherichia coli to d-Cysteine*
TLDR
In agreement with a role of the desulfhydrase in sulfur metabolism, yedO expression was induced under conditions of sulfate limitation and growth protection against d-cysteine in minimal medium was conferred by the simultaneous addition of isoleucines, leucine, and valine. Expand
Pyrophosphatase is essential for growth of Escherichia coli
The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ geneExpand
Metabolism of d-Aminoacyl-tRNAs inEscherichia coli and Saccharomyces cerevisiae Cells*
TLDR
The results indicate that an unexpected high number of d-amino acids can impair the bacterium growth through the accumulation ofd-Aminoacyl-tRNA molecules and that d-Tyr-tRNATyr deacylase has a specificity broad enough to recycle any of these molecules. Expand
Extracellular Production of Hydrogen Selenide Accounts for Thiol-assisted Toxicity of Selenite against Saccharomyces cerevisiae*
TLDR
It is shown that the lethal dose of selenite is reduced to the micromolar range by the presence of thiols in the growth medium, and the possibility that selenium exerts its toxicity through consumption of intracellular reduced glutathione, thus leading to severe oxidative stress is discussed. Expand
Widespread Distribution of Cell Defense against d-Aminoacyl-tRNAs*
Several l-aminoacyl-tRNA synthetases can transfer a d-amino acid onto their cognate tRNA(s). This harmful reaction is counteracted by the enzyme d-aminoacyl-tRNA deacylase. Two distinct deacylasesExpand
D-tyrosyl-tRNA(Tyr) metabolism in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae YDL219w (DTD1) gene, which codes for an amino acid sequence sharing 34% identity with the Escherichia coli D-Tyr-tRNA(Tyr) deacylase, was cloned, and its product wasExpand
Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.
TLDR
In Escherichia coli, two distinct lysyl-tRNA synthetase species are encoded by two genes: the constitutive lysS gene and the thermoinducible lysU gene, which show 79% and 88% identity, respectively. Expand
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