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Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.
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Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
In the DNP system, the specificity of the reaction was assessed by inhibition with hapten. The reaction of immune serum against DNP with DNP-protein, adsorbed to the tubes, was completely inhibitedExpand
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Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.
Alkaline phosphatase from calf intestinal mucosa has been conjugated to a protein antigen, rabbit IgG. Such conjugates, prepared by glutardialdehyde, have been used in a competitive solid phaseExpand
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Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy
As tested in vitro in a microcytotoxicity assay, purified peripheral blood lymphocytes from patients with carcinoma of the urinary bladder have a specific destructive effect on primary cultures ofExpand
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Malaria and the immune system in humans.
Malaria infection gives rise to host responses which are regulated by both the innate and adaptive immune system as well as by environmental factors. Acquired immunity is both speciesandExpand
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Sera from 30 children, suffering from ulcerative colitis, were examined for the presence of antibodies capable of reacting with antigens of normal human tissue. It was possible to demonstrate thatExpand
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Pf155/RESA antigen is localized in dense granules of Plasmodium falciparum merozoites.
Immunoelectron microscopy demonstrated the presence of Pf155/RESA in dense granules of Plasmodium falciparum merozoites rather than in micronemes as previously suggested. Since the dense granules areExpand
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IgE elevation and IgE anti‐malarial antibodies in Plasmodium falciparum malaria; association of high IgE levels with cerebral malaria
In the course of studying immunoregulation in human Plasmodium falciparum malaria we have investigated IgE levels and IgE anti‐plasmodial antibodies in children and adults from areas of high malariaExpand
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Production by activated human T cells of interleukin 4 but not interferon-gamma is associated with elevated levels of serum antibodies to activating malaria antigens.
T cells play a crucial role in antibody-mediated and antibody-independent immunity against Plasmodium falciparum malaria. Therefore, a vaccine immunogen should include parasite-derived B- and T-cellExpand
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Surface display compared to periplasmic expression of a malarial antigen in Salmonella typhimurium and its implications for immunogenicity.
Two different expression systems were investigated for the production of an 80 amino acid polypeptide, M3, from the C-terminus of the Plasmodium falciparum blood stage antigen Pf155/RESA in anExpand
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