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Rhamnogalacturonan-II, a Pectic Polysaccharide in the Walls of Growing Plant Cell, Forms a Dimer That Is Covalently Cross-linked by a Borate Ester
Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide present in the walls of growing plant cells. We now report that RG-II, released by endopolygalacturonase treatment of theExpand
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Structural characterization of red wine rhamnogalacturonan II.
The pectic polysaccharide rhamnogalacturonan II (RG-II), which accounts for approximately 20% of the ethanol-precipitable polysaccharides in red wine, has been isolated from wine polysaccharides byExpand
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The Plant Cell Wall Polysaccharide Rhamnogalacturonan II Self-assembles into a Covalently Cross-linked Dimer*
The location of the 1:2 borate-diol ester cross-link in the dimer of the plant cell wall polysaccharide rhamnogalacturonan II (RG-II) has been determined. The ester cross-links the apiofuranosylExpand
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Polysaccharides from grape berry cell walls. Part I: tissue distribution and structural characterization of the pectic polysaccharides
Abstract Buffer-soluble arabinogalactan-proteins (AGPs) and pectins from grape berry skin and pulp tissues have been isolated and their structure has been partly determined. Pectic polysaccharidesExpand
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The polysaccharides of red wine: total fractionation and characterization
Abstract Ethanol-precipitated red wine polysaccharides were fractionated by a combination of anion-exchange, size-exclusion and affinity chromatography steps. This comprehensive fractionation allowedExpand
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Characterization of five type II arabinogalactan-protein fractions from red wine of increasing uronic acid content.
Five arabinogalactan-protein conjugates (AGP) were separated from red wine by two successive anion-exchange chromatography steps and further purified to apparent homogeneity by affinity andExpand
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A Saccharomyces mannoprotein that protects wine from protein haze
Abstract A Saccharomyces cerevisiae yeast mannoprotein which protected wine from protein haze spoilage was isolated from a red wine and purified by a combination of anion exchange, Concanavalin A,Expand
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Structural characterization of the pectic polysaccharide rhamnogalacturonan II: evidence for the backbone location of the aceric acid-containing oligoglycosyl side chain.
Monomeric rhamnogalacturonan II (mRG-II) was isolated from red wine and the reducing-end galacturonic acid of the backbone converted to L-galactonic acid by treatment with NaBH4. The resultingExpand
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Determination of the neutral and acidic glycosyl-residue compositions of plant polysaccharides by GC-EI-MS analysis of the trimethylsilyl methyl glycoside derivatives
A method, using GC-EI-MS analysis of the trimethylsilyl methyl glycoside derivatives, has been developed to identify the neutral and acidic glycosyl-residue compositions of plant polysaccharides.Expand
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Involvement of pectin methyl-esterase during the ripening of grape berries: partial cDNA isolation, transcript expression and changes in the degree of methyl-esterification of cell wall pectins.
Grape berries (Vitis vinifera L., cv Ugni blanc) were harvested at 12 different weeks of development in 1996 and 1997. Ripening was induced at veraison, the crucial stage of berry softening, and wasExpand
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