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MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimentalExpand
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Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
BackgroundSalmonid fishes are among the most widely studied model fish species but reports on systematic evaluation of reference genes in qRT-PCR studies is lacking.ResultsThe stability of sixExpand
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Novel production of Atlantic salmon (Salmo salar) protein based on combined replacement of fish meal and fish oil with plant meal and vegetable oil blends
The aim of the present study was to combine maximum replacement of fish meal and fish oil with plant ingredients in feed for Atlantic salmon, in order to gain a sound and sustainable net fish proteinExpand
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Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels.
Three populations of brown trout (Salmo trutta) exposed to different metal levels in their natural environments, were studied with respect to antioxidants metallothionein (MT), superoxide dismutaseExpand
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The need for transparency and good practices in the qPCR literature
TLDR
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Expand
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Effects of atrazine on hepatic metabolism and endocrine homeostasis in rainbow trout (Oncorhynchus mykiss).
The herbicide atrazine (ATZ) is one of the most widely used pesticides in the world and is now under scrutiny for its alleged capacity to disrupt the endocrine system. Exhibiting negligibleExpand
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Characterization and expression of digestive neutral lipases during ontogeny of Atlantic cod (Gadus morhua).
The major neutral lipase excreted by the pancreas in fish, is bile activated lipase (BAL). Here we present evidence that cod have a functional BAL and a non-functional pancreatic lipase relatedExpand
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Gene expression of fatty acid‐binding proteins, fatty acid transport proteins (cd36 and FATP) and β‐oxidation‐related genes in Atlantic salmon (Salmo salar L.) fed fish oil or vegetable oil
Relative gene expression pattern of fatty acid transport proteins (FATP and cd36), intracellular fatty acid-binding proteins (FABP3, FABP10 and FABP11), β-oxidation-related genes [carnitine palmitoylExpand
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mRNA expression of antioxidant enzymes (SOD, CAT and GSH-Px) and lipid peroxidative stress in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water during smoltification.
The mRNA levels of three antioxidant genes, Cu/Zn superoxide dismutase (SOD), catalase (CAT) and phospholipid hydroperoxide glutathione peroxidase (GSH-Px), were quantified with real-time qRT-PCR inExpand
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Transcriptional effects of PFOS in isolated hepatocytes from Atlantic salmon Salmo salar L.
The main aim of the current in vitro experiment was to search for makers for PFOS exposure in isolated hepatocytes from Atlantic salmon Salmo salar, based on genes responding to PFOS exposure inExpand
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