• Publications
  • Influence
High resolution two-dimensional electrophoresis of proteins.
  • P. O'Farrell
  • Medicine, Chemistry
  • The Journal of biological chemistry
  • 25 May 1975
A technique has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. Due to its resolution and sensitivity, this technique is a powerful tool for theExpand
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High resolution two-dimensional electrophoresis of basic as well as acidic proteins
Abstract A previously described two-dimensional electrophoresis procedure (O'Farrell, 1975) combined isoelectric focusing and sodium dodecylsulfate slab gel electrophoresis to give high resolution ofExpand
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Distinct molecular mechanism regulate cell cycle timing at successive stages of Drosophila embryogenesis.
The conserved regulators of cell cycle progression--Cyclins, Cdc2 kinase, and String phosphatase (Cdc25)--accommodate multiple modes of regulation during Drosophila embryogenesis. During cell cyclesExpand
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The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing
Many metazoan cells can take up exogenous double-stranded (ds) RNA and use it to initiate an RNA silencing response, however, the mechanism for this uptake is ill-defined. Here, we identify theExpand
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Comparative genomics of the eukaryotes.
A comparative analysis of the genomes of Drosophila melanogaster, Caenorhabditis elegans, and Saccharomyces cerevisiae-and the proteins they are predicted to encode-was undertaken in the context ofExpand
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Identification of Drosophila Gene Products Required for Phagocytosis of Candida albicans
Phagocytosis is a highly conserved aspect of innate immunity. We used Drosophila melanogaster S2 cells as a model system to study the phagocytosis of Candida albicans, the major fungal pathogen ofExpand
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The transcription factor E2F is required for S phase during Drosophila embryogenesis.
Overexpression of the E2F-1 cDNA in mammalian cells disrupts normal control of the cell cycle and drives cells into S phase. Whereas eliminating E2F activity would test its inferred involvement inExpand
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Progression of the cell cycle through mitosis leads to abortion of nascent transcripts
Nascent transcripts of the Drosophila Ubx gene were detected by in situ hybridization. Following onset of expression, the progress of RNA polymerase (1.4 kb/min) across the gene was visualized as theExpand
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Triggering the all-or-nothing switch into mitosis.
  • P. O'Farrell
  • Biology, Medicine
  • Trends in cell biology
  • 1 December 2001
The past decade of cell cycle investigations has identified many roads not taken. The kinase that drives mitosis can be modulated by cyclins, by activating phosphorylation, by inhibitoryExpand
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The three postblastoderm cell cycles of Drosophila embryogenesis are regulated in G2 by string
The string (stg) locus of Drosophila encodes a factor that is thought to trigger mitosis by activating the p34cdc2 protein kinase. stg is required for mitosis early in development and is transcribedExpand
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