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Thermofluor-based high-throughput stability optimization of proteins for structural studies.
Production of proteins well suited for structural studies is inherently difficult and time-consuming. Protein sample homogeneity, stability, and solubility are strongly correlated with the proteins'Expand
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Monitoring Drug Target Engagement in Cells and Tissues Using the Cellular Thermal Shift Assay
Drug Targeting Drug efficacy depends on the extent of binding to a cellular target (often a protein) with adverse effects caused by excessive target binding or by off-target binding. Engagement of aExpand
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Ribonucleotide reductases.
Ribonucleotide reductases (RNRs) transform RNA building blocks to DNA building blocks by catalyzing the substitution of the 2'OH-group of a ribonucleotide with a hydrogen by a mechanism involvingExpand
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Structure and function of the radical enzyme ribonucleotide reductase.
Ribonucleotide reductases (RNRs) catalyze all new production in nature of deoxyribonucleotides for DNA synthesis by reducing the corresponding ribonucleotides. The reaction involves the action of aExpand
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The Crystal Structure of the Human Toll-like Receptor 10 Cytoplasmic Domain Reveals a Putative Signaling Dimer*
The Toll/interleukin-1 receptor (TIR) domain is a highly conserved signaling domain found in the intracellular regions of Toll-like receptors (TLRs), in interleukin-1 receptors, and in severalExpand
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Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli
Escherichia coli is one of the most widely used vehicles to overexpress membrane proteins (MPs). Currently, it is not possible to predict if an overexpressed MP will end up in the cytoplasmicExpand
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New insight into the structure and function of the alternative oxidase.
The alternative oxidase is a ubiquinol oxidase found in plant mitochondria, as well as in the mitochondria of some fungi and protists. It catalyzes a cyanide-resistant reduction of oxygen to waterExpand
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Crystal structure of human leukotriene A4 hydrolase, a bifunctional enzyme in inflammation
Leukotriene (LT) A4 hydrolase/aminopeptidase (LTA4H) is a bifunctional zinc enzyme that catalyzes the biosynthesis of LTB4, a potent lipid chemoattractant involved in inflammation, immune responses,Expand
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Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization
Ribonucleotide reductase (RR) is an αnβn (RR1–RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required forExpand
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The cellular thermal shift assay for evaluating drug target interactions in cells
Thermal shift assays are used to study thermal stabilization of proteins upon ligand binding. Such assays have been used extensively on purified proteins in the drug discovery industry and inExpand
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