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A Bruno-like gene is required for stem cell maintenance in planarians.
It is indicated that neoblasts lacking Bruli can respond to wound stimuli and generate progeny that can form blastemas and differentiate; yet, they are unable to self-renew, suggesting that Bruli is required for stem cell maintenance. Expand
Double-stranded RNA specifically disrupts gene expression during planarian regeneration.
The ability to eliminate gene function in a regenerating organism such as the planarian overcomes previous experimental limitations and opens the study of animal regeneration to unprecedented levels of molecular detail. Expand
nanos function is essential for development and regeneration of planarian germ cells
A conserved function of nanos is revealed in germ cell development in planarians and it is suggested that these animals will serve as useful models for dissecting the molecular basis of epigenetic germ cell specification. Expand
Planarian homologs of netrin and netrin receptor are required for proper regeneration of the central nervous system and the maintenance of nervous system architecture
RNAi analyses indicate that Smed-netR (netrin receptor) and SMed-netrin2 are required for proper CNS regeneration and that S med-netsR may mediate the response to Smed -netrin 2. Expand
The Schmidtea mediterranea database as a molecular resource for studying platyhelminthes, stem cells and regeneration
The characterization of ∼3000 non-redundant cDNAs from a clonal line of the planarian Schmidtea mediterranea, and the ability of abrogating gene expression in planarians using RNA interference technology, pave the way for a systematic study of the remarkable biological properties displayed by Platyhelminthes. Expand
In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea
It is shown that a short bleaching step in formamide dramatically enhances signal intensity of WISH and FISH and that azide most effectively quenches peroxidase activity between rounds of development for multicolor FISH experiments. Expand
Regeneration and maintenance of the planarian midline is regulated by a slit orthologue.
It is suggested that Smed-slit acts not only as a repulsive cue required for proper midline formation during regeneration but that it may also act to regulate the behavior of neural precursors at the midline in intact planarians. Expand
The planarian Schmidtea mediterranea as a model for epigenetic germ cell specification: analysis of ESTs from the hermaphroditic strain.
To develop molecular reagents for studying epigenetic germ cell specification, 27,161 ESTs from two developmental stages of the hermaphroditic strain of S. mediterranea are sequenced; this collection of ESTs represents approximately 10,000 unique transcripts and will be an important resource for annotating the planarian genome and studying this animal's amazing regenerative abilities. Expand
Bromodeoxyuridine specifically labels the regenerative stem cells of planarians.
The ability to label specifically the regenerative stem cells, combined with the recently described use of double-stranded RNA to inhibit gene expression in the planarian, should serve to reignite interest in the flatworm as an experimental model for studying the problems of metazoan regeneration and the control of stem cell proliferation. Expand
Genome-Wide Analyses Reveal a Role for Peptide Hormones in Planarian Germline Development
Genomic/peptidomic analyses of the planarian Schmidtea mediterranea identifies >200 neuropeptides and uncovers a conserved neuropeptide required for proper maturation and maintenance of theExpand