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Radioimmunoassay of 3α-hydroxy-5α-pregnan-20-one in rat and human plasma
Abstract A radioimmunoassay for measuring 3α-hydroxy-5α-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3α-hydroxy-20-oxo-5α-pregnan-11α-ylExpand
Enzyme immunoassay of cortisol in human plasma using penicillinase as label.
Although employing immunoassay plates for separation of antigen-antibody complex from the free components was less time consuming, the slope and sensitivity of the standard curve were improved by the addition of goat anti-rabbit gamma globulin for precipitating the complex. Expand
Radioimmunoassay of 3 alpha-hydroxy-5 alpha-pregnan-20-one in rat and human plasma.
3 alpha-Hydroxy-5 alpha-pregnan-20-one was detected in plasma from female rats on the day of estrus and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml, which was highly correlated with plasma progesterone levels. Expand
Analysis of metabolites of progesterone in bovine liver, kidney, kidney fat, and milk by high performance liquid chromatography.
This study demonstrates for the first time the utility of HPLC for the rapid analysis of a complete mixture of metabolites of progesterone from crude extracts of mammalian tissues. Expand
Synthesis of new steroid haptens for radioimmunoassay. Part IV. 3-O-carboxymethyl ether derivatives of estrogens. Specific antisera for radioimmunoassay of estrone, estradiol-17β, and estriol
The syntheses of 3-O-carboxymethyl ether derivatives of estrone, estradiol-17beta, and estriol and the preparation of their bovine serum albumin (BSA) conjugates are described and the previous concept that specific antisera for estrogens cannot be obtained by employing estrogens derivatized at the 3-position is unfounded. Expand
Synthesis of new steroid haptens for radioimmunoassay. Part III. 15β-carboxyethylmercaptosteroid-bovine serum albumin conjugates. Specific antisera for radioimmunoassay of 5α-dihydrotestosterone,
Abstract The syntheses of 15β-carboxyethylmercapto-5α-dihydrotestosterone, 15β-carboxy-ethylmercapto-5α-androstane-3β, 17β-diol and 15β-carboxyethylmercapto-5α-androstane-3α, 17β-diol and theExpand
Novel 2-methoxyestradiol analogues with antitumor activity.
Mechanism of action studies indicate that the novel 2-ME2 analogues cause Bcl-2 phosphorylation and activation of mitogen-activated protein kinase signaling pathways, which are consistent with antimitotics that inhibit the dynamic instability of tubulin and initiate apoptosis. Expand
Enzyme immunosorbant assay of oestradiol in unextracted plasma using penicillinase as label.
An enzyme-linked immunosorbant assay (ELISA) for measuring oestradiol directly in plasma without extraction utilizing antibodies raised against oestradiol-3-(O-carboxymethyl) ether-bovine serumExpand
Synthesis and antimitotic activity of novel 2-methoxyestradiol analogs
Structural modifications investigated include introduction of additional unsaturation in rings B and D; inversion at C-13; and substitution at the C-2, C-15,C-16, and C-7 alpha positions. Expand
Synthesis of 2-methoxy and 4-methoxy equine estrogens
Nucleophilic displacement of iodine with methoxide ion was carried out as described earlier with simultaneous aromatization of the B ring leading to 4- and 2-methoxyequilenin derivatives. Expand