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High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
The ability to transform routinely plastids of land plants opens the way to manipulate the process of photosynthesis and to incorporate novel genes into the plastid genome of crops.
The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
The newpPZP Agrobacterium binary vectors are versatile, relatively small, stable and fully sequenced, allowing their use inAgrobacteria strains with different drug resistance markers.
The two RNA polymerases encoded by the nuclear and the plastid compartments transcribe distinct groups of genes in tobacco plastids
Distinct NEP and PEP promoters reported here provide a general mechanism for group‐specific gene expression through recognition by the two RNA polymerases.
Stable transformation of plastids in higher plants.
- Z. Sváb, P. Hajdukiewicz, P. Maliga
- BiologyProceedings of the National Academy of Sciences…
- 1 November 1990
Stable genetic transformation of the plastid genome is reported in a higher plant, Nicotiana tabacum, obtained after bombardment of leaves with tungsten particles coated with pZS148 plasmid DNA.
Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants.
By deleting the gene for the essential beta subunit of the tobacco E.coli sigma70‐type promoters, this work has established the existence of a second plastid transcription system which does not utilize E. coli‐like promoters and preferentially transcribes genetic system genes rather than photosynthetic genes.
Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes
It is shown here that tobacco chloroplasts contain a protein complex of 550 kDa consisting of at least three of the ndh gene products: NdhI, NdhJ and NdhK, indicating that the Ndh complex is dispensible for plant growth under optimal growth conditions.
Plastid transformation in higher plants.
- P. Maliga
- Biology, EngineeringAnnual review of plant biology
- 29 April 2004
This review provides an overview of the technology for the genetic modification of the plastid genome including: vectors, marker genes and gene design, the use of gene knockouts and over-expression to probe plastsid function and the application of site-specific recombinases for excision of target DNA.
Callus Induction and Plant Regeneration from Mesophyll Protoplasts of Nicotiana sylvestris
Complementarity of the 16S rRNA penultimate stem with sequences downstream of the AUG destabilizes the plastid mRNAs.
It is reported here that the phage T7g10 5'-UTR and DB region promotes accumulation of NPTII up to approximately 16% of total soluble leaf protein (TSP).
Streptomycin-resistant plants from callus culture of haploid tobacco.
Conditions are worked out for the isolation of streptomycin-resistant tobacco cell lines, from which diploid fertile plants carrying the streptomecin- resistant character, were regenerated and seedlings grown from seeds of self-fertilized resistant Str-r1 plants were resistant.