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Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology
The pPMQAK1 shuttle vector allows the use of the growing numbers of BioBrick parts in many prokaryotes, and the other tools herein implemented facilitate the development of new parts and systems in cyanobacteria. Expand
Hydrogenases and Hydrogen Metabolism of Cyanobacteria
This review summarizes the molecular knowledge about cyanobacterial hupSL and hoxFUYH, their corresponding gene products, and their accessory genes before finishing with an applied aspect—the use of cyanobacteria in a biological, renewable production of the future energy carrier molecular hydrogen. Expand
Development of suitable photobioreactors for CO2 sequestration addressing global warming using green algae and cyanobacteria.
The present paper deals with the photobioreactors of different geometry available for biomass production and focuses on the hybrid types of reactors (integrating two reactors) which can be used for overcoming the bottlenecks of a single Photobioreactor. Expand
Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria
This work uses the NHM5 strain of Nostoc punctiforme ATCC 29133 to compare and improve existing phenol based chemistry and procedures for RNA extraction, and identifies and explores strategies for improved and lower cost high quality RNA isolation from cyanobacteria. Expand
Synthetic biology in cyanobacteria engineering and analyzing novel functions.
With this chapter, the synthetic biologist is given an overview of existing biological parts, tools and protocols for the genetic engineering, and molecular analysis of cyanobacteria for Synthetic Biology applications. Expand
Cyanobacterial hydrogenases: diversity, regulation and applications.
In cyanobacteria, the genes that are thought to affect hydrogenases pleiotropically (hyp), as well as the genes presumably encoding the hydrogenase-specific endopeptidases (hupW and hoxW) have been identified and characterized, and NtcA and LexA have been implicated in the transcriptional regulation of the uptake and the bidirectional enzyme respectively. Expand
Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium
The artificial constitutive promoters herein characterized can be utilized for expression in cyanobacteria, as demonstrated for LacI, and two well-repressed Ptrc 2O promoters were highly active when tested in absence of LacI in Synechocystis. Expand
Recent trends on the development of photobiological processes and photobioreactors for the improvement of hydrogen production
Hydrogen production through biological routes is promising because they are environmentally friendly. Hydrogen production through biophotolysis or photofermentation is usually a two stage process. InExpand
Cyanobacterial H2 production — a comparative analysis
It was shown that the hydrogen uptake activity is linked to the nitrogenase activity, whereas the hydrogen evolution activity of the bidirectional hydrogenase is not dependent or even related to diazotrophic growth conditions. Expand
Sequence Variation of the tRNALeuIntron as a Marker for Genetic Diversity and Specificity of Symbiotic Cyanobacteria in Some Lichens
The genetic diversity of Nostoc symbiont in situ is not a diverse community of cyanobionts but, rather, one Nostoc strain, indicating that each lichen thallus is colonized only once or that there is a high degree of specificity. Expand