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Methods for Measurement of Intermolecular NOEs by Multinuclear NMR Spectroscopy: Application to a Bacteriophage λ N-Peptide/boxB RNA Complex
New pulse schemes for recording intermolecular NOEs in a molecular complex consisting of 15N,13C labeled and unlabeled components are presented. The pulse sequences select for magnetizationExpand
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Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53.
The interaction between the amino-terminal transactivation domain (TAD) of p53 and TFIIH is directly correlated with the ability of p53 to activate both transcription initiation and elongation. WeExpand
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NMR Structure of the Bacteriophage λ N Peptide/boxB RNA Complex: Recognition of a GNRA Fold by an Arginine-Rich Motif
The structure of the complex formed by the arginine-rich motif of the transcriptional antitermination protein N of phage lambda and boxB RNA was determined by heteronuclear magnetic resonanceExpand
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Structural and functional evidence that Rad4 competes with Rad2 for binding to the Tfb1 subunit of TFIIH in NER
XPC/Rad4 (human/yeast) recruits transcription faction IIH (TFIIH) to the nucleotide excision repair (NER) complex through interactions with its p62/Tfb1 and XPB/Ssl2 subunits. TFIIH then recruitsExpand
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Unusual dynamics and pKa shift at the active site of a lead dependent ribozyme
Heteronuclear NMR spectroscopy has been used to probe the structure and dynamics of a lead-dependent ribozyme known as the leadzyme. The pKa's for all adenine bases in the leadzyme were measured andExpand
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Solution structure of bovine neutrophil beta-defensin-12: the peptide fold of the beta-defensins is identical to that of the classical defensins.
The solution structure is reported for bovine neutrophil beta-defensin-12 (BNBD-12), a member of the beta-defensin family of antimicrobial peptides. Structural constraints in the form ofExpand
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Structure of a (Cys3His) zinc ribbon, a ubiquitous motif in archaeal and eucaryal transcription
Transcription factor IIB (TFIIB) is an essential component in the formation of the transcription initiation complex in eucaryal and archaeal transcription. TFIIB interacts with a promoter complexExpand
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NMR structure of the active conformation of the Varkud satellite ribozyme cleavage site
Substrate cleavage by the Neurospora Varkud satellite (VS) ribozyme involves a structural change in the stem-loop I substrate from an inactive to an active conformation. We have determined the NMRExpand
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A stable mercury-containing complex of the organomercurial lyase MerB: catalysis, product release, and direct transfer to MerA.
Bacteria isolated from organic mercury-contaminated sites have developed a system of two enzymes that allows them to efficiently convert both ionic and organic mercury compounds to the less toxicExpand
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Preparation of 13C and 15N labelled RNAs for heteronuclear multi-dimensional NMR studies.
A procedure is described for the efficient preparation of isotopically enriched RNAs of defined sequence. Uniformly labelled nucleotide 5'triphosphates (NTPs) were prepared from E.coli grown on 13CExpand
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