The genome sequence of Drosophila melanogaster.
The nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome is determined using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map.
The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A
Isolation and characterization of vasa genomic and complementary DNA clones show that the transcript is abundant in the female germ line and early embryos only.
Autism-related deficits via dysregulated eIF4E-dependent translational control
Hyperconnectivity of neuronal circuits due to increased synaptic protein synthesis is thought to cause autism spectrum disorders (ASDs). The mammalian target of rapamycin (mTOR) is strongly…
Translational repressor bruno plays multiple roles in development and is widely conserved.
The isolation of a cDNA encoding Bru using a novel approach to the expression cloning of an RNA-binding protein, and the identification of previously described mutants in the arrest (aret)-locus as mutants in Bru indicate that Bru regulates multiple mRNAs involved in female and male gametogenesis as well as early in embryogenesis.
vasa is required for GURKEN accumulation in the oocyte, and is involved in oocyte differentiation and germline cyst development.
A new null mutation of vasa was generated, which deletes the entire coding region of the vasa gene and revealed roles for vasa in oocyte differentiation, anterior-posterior egg chamber patterning, and dorsal-ventral follicle patterned, in addition to its better-characterized functions in posterior embryonic patterning and pole cell specification.
Posterior localization of vasa protein correlates with, but is not sufficient for, pole cell development.
It is indicated that vasa protein, when properly localized, is not sufficient for induction of pole cell development, and that at least the tudor and valois wild-type functions are also required specifically for this process.
Germline development in vertebrates and invertebrates
The unique properties of germ cells, the initial determination of germcell fates, the maintenance of germ cell identity, the migration of germcells to the somatic gonadal primordia and the proliferation of germ Cells during development in vertebrates and invertebrates are reviewed.
mRNA helicases: the tacticians of translational control
Growing evidence suggests that other helicases are also important for translation initiation and may promote the scanning processivity of the 40S subunit, synergize with eIF4A to 'melt' secondary structures or facilitate translation of a subset of mRNAs.
Localization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activities.
It is found that localization of vasa to the perinuclear nuage is abolished in most vas alleles, but is unaffected by mutations in four genes required upstream for its pole plasm localization, suggesting that the formation of the nuage depends upon vas function.