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Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine.
This work engineered and optimized a synthetic pathway for de novo biosynthesis of β-alanine and its subsequent conversion into 3HP using a novel β-Alanine-pyruvate aminotransferase discovered in Bacillus cereus. Expand
Effects of Temperature, Salinity, and Medium Composition on Compatible Solute Accumulation byThermococcus spp
An unexpected ability of T. litoralis to scavenge suitable components from the medium and to use them as compatible solutes is revealed, which is likely to be derived from peptone-containing medium. Expand
Thermostabilization of Proteins by Diglycerol Phosphate, a New Compatible Solute from the HyperthermophileArchaeoglobus fulgidus
  • P. Lamosa, A. Burke, +7 authors Helena Santos
  • Biology, Medicine
  • Applied and Environmental Microbiology
  • 1 May 2000
The data show that diglycerol phosphate is a potentially useful protein stabilizer in biotechnological applications and its different behaviors are discussed in the light of the known structural features of rubredoxins. Expand
Pseudovitamin B 12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
The chemical characterisation of this corrinoid‐like molecule is reported on, which differs from cobalamin in the α ‐ligand, where it has adenine instead of 5,6‐dimethylbenzimidazole bound in aα ‐glycosidic linkage to C‐1 of ribose. Expand
Molecular Identification of N-Acetylaspartylglutamate Synthase and β-Citrylglutamate Synthase*
The purpose of the present work was to determine the identity of the enzymes that synthesize N-acetylaspartylglutamate (NAAG), the most abundant dipeptide present in vertebrate central nervous systemExpand
The Physiological Role, Biosynthesis, and Mode of Action of Compatible Solutes from (Hyper)Thermophiles
Many thermophiles and hyperthermophiles (from now on designated (hyper)thermophiles) have been isolated from both fresh water and seawater sources. Compatible solutes must be highly soluble and theyExpand
Occurrence of 1-Glyceryl-1-myo-Inosityl Phosphate in Hyperthermophiles
The newly discovered compound, 1-glyceryl-1-myo-inosityl phosphate, appears to play a double role in osmo- and thermoprotection, since its intracellular pool increased primarily in response to a combination of osmotic and heat stresses. Expand
Identification of glucoselysine-6-phosphate deglycase, an enzyme involved in the metabolism of the fructation product glucoselysine.
GfrF and GfrE serve to metabolize glycation products formed from lysine and glucose (fructoselysine) or fructose (glucoselysine), via their 6-phospho derivatives, which are presumably formed by the putative phosphotransferase system encoded by gfrA-gfrD. Expand
Butyrate-rich Colonic Microenvironment Is a Relevant Selection Factor for Metabolically Adapted Tumor Cells*
The data showed that butyrate regulates the expression of VEGF and its receptor KDR at the transcriptional level potentially through FoxM1, resulting in the generation of a functional V EGF:KDR autocrine growth loop, which shows that abutyrate-rich microenvironment may select for tumor cells that are able to metabolize butyrates, which are also phenotypically more aggressive. Expand
Bifunctional CTP:Inositol-1-Phosphate Cytidylyltransferase/CDP-Inositol:Inositol-1-Phosphate Transferase, the Key Enzyme for Di-myo-Inositol-Phosphate Synthesis in Several (Hyper)thermophiles
Genome analysis showed homologous genes in all organisms known to accumulate DIP and for which genome sequences were available, and the stereochemical configuration of all the metabolites involved in DIP synthesis was established by NMR analysis. Expand