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Structure and mechanism of Na,K-ATPase: functional sites and their interactions.
Evidence is related on functional sites of Na,K-ATPase for the substrate (ATP), the essential cofactor (Mg(2+) ions), and the transported cations (Na(+) and K(+)) to the molecular structure to address the central questions of mechanism of active cation transport by all P-type cation pumps. Expand
Purification and characterization of (Na+ plus K+ )-ATPase. 3. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate.
Analysis of the protein composition by sodium dodecylsulphate gel electrophoresis and determination of the capacities for binding of ATP and ouabain and for sodium-dependent phosphorylation show that the procedures lead to a true purification of the enzyme. Expand
Importance for Absorption of Na+ from Freshwater of Lysine, Valine and Serine Substitutions in the α1a-Isoform of Na,K-ATPase in the Gills of Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon
Together, the three substitutions in the α1a-isoform of Na,K-ATPase act to promote binding of Na+ over K+ from the cytoplasm, to reduce the Na+/ATP ratio and the work done in one Na, K pump cycle of active Na+ transport against the steep gradient from freshwater to blood. Expand
Mechanism of the Na+, K+ pump. Protein structure and conformations of the pure (Na+ +K+)-ATPase.
Isolation and characterization of the components of the sodium pump.
Conformational changes of renal sodium plus potassium ion-transport adenosine triphosphatase labeled with fluorescein.
Five conformational changes of purified renal sodium plus potassium ion-transport adenosine triphosphatase (ATP phosphohydrolase, EC labeled with fluorescein isothiocyanate are studied, finding that low and high affinity for Mg2+ alternates as the enzyme turns over, Mg 2+ may be bound and released sequentially during the catalytic cycle. Expand
Modulation of plasma membrane H+-ATPase from oat roots by lysophosphatidylcholine, free fatty acids and phospholipase A2
Plasma membrane vesicles were purified from 8-day-old oat (Avena sativa L. cv. Brighton) roots in an aqueous polymer two-phase system. The plasma membranes possessed high specific ATPase activity [caExpand
Structural basis for E1–E2 conformational transitions in Na, K-pump and Ca-pump proteins
The intermediary steps of the pump reactions and their relationship to cation translocation have been examined in detail and the combined X-ray crystallographic structure at high resolution and as paradigm for interpretation of this information is used. Expand
Identification of Asp804 and Asp808 as Na+ and K+ coordinating residues in α‐subunit of renal Na,K‐ATPase
Demonstration of alternate interactions of Na+ or K+ with Asp804 and Asp808 support the notion of cation binding in a ping‐pong sequence in catalytic models of Na,K‐pumping. Expand