• Publications
  • Influence
T-DNA as an Insertional Mutagen in Arabidopsis
Forward genetics begins with a mutant phenotype and asks the question “What is the genotype?” that is, what is the sequence of the mutant gene causing the altered phenotype? Reverse genetics beginsExpand
  • 783
  • 80
  • PDF
Genetic evidence for the in planta role of phloem-specific plasma membrane sucrose transporters.
A major question in plant physiology is how the large amount of sucrose made in leaves is transported to the rest of the plant. Although physiological, biochemical, and anatomical investigations haveExpand
  • 324
  • 30
  • PDF
The Arabidopsis knockout facility at the University of Wisconsin-Madison.
As of this writing the Arabidopsis genome is 97% sequenced with only small portions of the highly repetitive regions within centromeres and telomeres remaining. The identification of approximatelyExpand
  • 217
  • 27
MEKK1 Is Required for flg22-Induced MPK4 Activation in Arabidopsis Plants1[C][W]
The Arabidopsis (Arabidopsis thaliana) gene MEKK1 encodes a mitogen-activated protein kinase kinase kinase that has been implicated in the activation of the map kinases MPK3 and MPK6 in response toExpand
  • 280
  • 21
  • PDF
An Arabidopsis Mitogen-Activated Protein Kinase Kinase Kinase Gene Family Encodes Essential Positive Regulators of Cytokinesis Article, publication date, and citation information can be found at
The signal transduction pathways that control cytokinesis in plants are largely uncharacterized. Here, we provide genetic evidence that mitogen-activated protein kinase kinase kinases (MAPKKKs) playExpand
  • 152
  • 15
  • PDF
Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport.
The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis inExpand
  • 278
  • 13
  • PDF
Isolation of human sequences that replicate autonomously in human cells.
We have isolated a heterogeneous collection of human genomic sequences which replicate autonomously when introduced into human cells. The novel strategy for the isolation of these sequences involvedExpand
  • 229
  • 9
The WiscDsLox T-DNA collection: an arabidopsis community resource generated by using an improved high-throughput T-DNA sequencing pipeline
We have developed a new community resource, called the WiscDsLox collection, for performing reverse-genetic analysis in arabidopsis. This resource is composed of 10,459 T-DNA lines generated usingExpand
  • 140
  • 9
Chromosomal translocations are a common phenomenon in Arabidopsis thaliana T-DNA insertion lines.
Ordered collections of Arabidopsis thaliana lines containing mapped T-DNA insertions have become an important resource for plant scientists performing genetic studies. Previous reports have indicatedExpand
  • 70
  • 7
  • PDF
Deletion of a Tandem Gene Family in Arabidopsis: Increased MEKK2 Abundance Triggers Autoimmunity when the MEKK1-MKK1/2-MPK4 Signaling Cascade Is Disrupted[C][W]
This work examines the MAP kinase cascade encoded by MEKK1, MKK1/MKK2, and MPK4, finding that deletion of the MEKK1/2/3 tandem gene family produces a wild-type plant and that the autoimmuneExpand
  • 36
  • 5
  • PDF