The Phaeodactylum genome reveals the evolutionary history of diatom genomes
- C. Bowler, A. Allen, I. Grigoriev
- 13 November 2008
Analysis of molecular divergence compared with yeasts and metazoans reveals rapid rates of gene diversification in diatoms, and documents the presence of hundreds of genes from bacteria, likely to provide novel possibilities for metabolite management and for perception of environmental signals.
A Model for Carbohydrate Metabolism in the Diatom Phaeodactylum tricornutum Deduced from Comparative Whole Genome Analysis
- P. Kroth, A. Chiovitti, C. Bowler
- BiologyPLoS ONE
- 9 January 2008
A detailed synthesis of carbohydrate metabolism in diatoms based on the genome sequences of Thalassiosira pseudonana and Phaeodactylum tricornutum is presented, which provides novel insights into acquisition of dissolved inorganic carbon and primary metabolic pathways of carbon in two different diats, which is of significance for an improved understanding of global carbon cycles.
Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes
- L. Zaslavskaia, J. C. Lippmeier, P. Kroth, A. Grossman, K. Apt
- 1 April 2000
It is demonstrated that a variety of selectable markers and reporter genes can be expressed in P. tricornutum, enhancing the potential of this organism for exploring basic biological questions and industrial applications.
Plastid proteome prediction for diatoms and other algae with secondary plastids of the red lineage
- Ansgar Gruber, G. Rocap, P. Kroth, E. Armbrust, T. Mock
- BiologyThe Plant Journal
- 6 January 2015
The plastids of ecologically and economically important algae from phyla such as stramenopiles, dinoflagellates and cryptophytes were acquired via a secondary endosymbiosis and are surrounded by three or four membranes and therefore represent the putative plastid proteomes of these algae.
Identification and characterization of a new conserved motif within the presequence of proteins targeted into complex diatom plastids.
- Oliver Kilian, P. Kroth
- BiologyThe Plant Journal
- 2 December 2004
It is demonstrated that treatment of the cells with Brefeldin A arrests protein transport into the diatom plastids suggesting that a vesicular transport step within the plastid membranes may occur.
Photoprotection capacity differs among diatoms: Possible consequences on the spatial distribution of diatoms related to fluctuations in the underwater light climate
- J. Lavaud, R. Strzepek, P. Kroth
- Environmental Science
- 1 May 2007
Estuarine species show a higher and more flexible capacity for photoprotection than oceanic and coastal species, and when exposed to excess light, the impairment of their photosynthetic capacity because of photoinhibition was reduced, resulting in maintenance of growth in a fluctuating light regime.
The regulation of carbon and nutrient assimilation in diatoms is significantly different from green algae.
- C. Wilhelm, C. Büchel, P. Kroth
- Environmental ScienceProtist
- 12 April 2006
Protein targeting into complex diatom plastids: functional characterisation of a specific targeting motif
- Ansgar Gruber, Sascha Vugrinec, F. Hempel, S. Gould, U. Maier, P. Kroth
- BiologyPlant Molecular Biology
- 5 May 2007
This comprehensive mutational analysis found that only the aromatic amino acids phenylalanine, tryptophan, tyrosine and the bulky amino acid leucine at the +1 position of the predicted signal peptidase cleavage site allow plastid import, as expected from the sequence comparison of native plastids targeting presequences of P. tricornutum and the cryptophyte Guillardia theta.
Algal genomes reveal evolutionary mosaicism and the fate of nucleomorphs
- B. Curtis, Goro Tanifuji, J. Archibald
- 28 November 2012
The nuclear genomes of the cryptophyte Guillardia theta and the chlorarachniophyte Bigelowiella natans are sequenced and extensive genetic and biochemical mosaicism is revealed, with both host- and endosymbiont-derived genes servicing the mitochondrion, the host cell cytosol, the plastid and the remnant endosYmbionT cytOSol of both algae.
In vivo characterization of diatom multipartite plastid targeting signals
- K. Apt, Lioudmila Zaslavkaia, P. Kroth
- BiologyJournal of Cell Science
- 1 November 2002
The data strongly support the hypothesis of a multi-step plastid targeting process in chromophytic algae and raises questions about the continuity of the ER and CER and the function of the latter in polypeptide trafficking.