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Agrobacterium tumefaciens-mediated genetic transformation of mungbean (Vigna radiata L. Wilczek) - a recalcitrant grain legume.
Agrobacterium tumefaciens-mediated high frequency genetic transformation of an Indian cowpea (Vigna unguiculata L. Walp.) cultivar and transmission of transgenes into progeny
The effect of TDZ on organogenesis and somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp)
TDZ-induced direct shoot organogenesis and somatic embryogenesis on cotyledonary node explants of lentil (Lens culinaris Medik.)
- G. Chhabra, Darshna Chaudhary, Madan Varma, Manish Sainger, P. K. Jaiwal
- Medicine, BiologyPhysiology and Molecular Biology of Plants
- 1 October 2008
An efficient and simple procedure for inducing high frequency direct shoot organogenesis and somatic embryogenesis in lentil from cotyledonary node explants in response to TDZ alone is reported and the possible role of stress in thidiazuron induced somatic embryos is discussed.
Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.)
- M. Yadav, Darshna Chaudhary, Manish Sainger, P. K. Jaiwal
- BiologyPlant Cell, Tissue and Organ Culture (PCTOC)
- 13 July 2010
For the first time successful recovery of fertile transgenic plants of sesame from cotyledon explants inoculated with Agrobacterium tumefaciens carrying a binary vector pCAMBIA2301 is reported, which can be used to transfer new traits in sesame for quantitative and qualitative improvement.
Plant regeneration from cotyledonary node explants of mungbean (Vigna radiata (L.) Wilczek)
Sexually-mature mungbean (Vigna radiata (L.) Wilczek) plants were efficiently regenerated from cotyledonary node explants. The explants were capable of directly developing multiple shoots on basal…
Iron uptake and transport in plants.
Agrobacterium tumefaciens mediated transfer of Phaseolus vulgaris α-amylase inhibitor-1 gene into mungbean Vigna radiata (L.) Wilczek using bar as selectable marker
Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and α-amylase inhibitor, have been developed for the first time and inheritance of both thetransgenes in most of the T1 lines was revealed.
Agrobacterium tumefaciens-mediated transformation of blackgram: An assessment of factors influencing the efficiency of uidA gene transfer
Various factors such as preculture and wounding of explants, manipulations in inoculation and co-cultivation conditions were found to play a significant role in influencing tissue competence, Agrobacterium virulence and compatibility of both, for achieving the maximum transformation frequencies.