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Functional Characterizations of Chemosensory Proteins of the Alfalfa Plant Bug Adelphocoris lineolatus Indicate Their Involvement in Host Recognition
TLDR
Three novel CSP genes (AlinCSP1-3) of the alfalfa plant bug Adelphocoris lineolatus are reported for the first time by screening the antennal cDNA library by identifying the types of sensilla in the antennae of the plant bug. Expand
Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera: Miridae)
TLDR
A cDNA library from the antennae of Apolygus lucorum provides valuable target genes to control the insect pests and indicates their potential olfactory functions in host seeking and mate finding of A. lucorum. Expand
[Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli].
TLDR
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), two primers were synthesized, which were complementary in a region, which gave rise to a recombinant plasmid pET(IT3R), which was transformed into E. coli BL21 (DE3). Expand
Deletion of Superoxide Dismutase Gene of Bombyx mori Nuclear Polyhedrosis Virus Affects Viral DNA Replication
TLDR
It was discovered that the sod gene was not essential for viral replication, and studies on growth of budded virus in BmN cells and superoxide dismutase and catalase activities in vivo after dsBmNPV infection showed that the titer of dSOD decreased obviously comparing to wild type Bm NPV. Expand
[Expression of phytase gene in Bombyx mori].
TLDR
The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied and expressed in silkworm larvae and pupae were studied. Expand
[Package of Bombyx mori nuclear polyhedrosis virus with heterogoneous polyhedring protein].
TLDR
It is confirmed that polyhedring of AcMNPV can be not only high-level expressed in BmN cells, But can recognize recombinant BmNPV DNA and assemble them to polyhedra. Expand
Expression of the Arrowhead Proteinase Inhibitor B (API) Gene in the Silkworm Larvae and Pupae.
TLDR
The recombinant API (rAPI) showed strong inhibitive activity on bovine pancreas trypsin and the preliminarily purified rAPI showed its molecular weight to be about 19 kD. Expand
Signal peptides leading secretory expression of foreign genes in insect expression system
TLDR
expression in larvae showed that the signal peptides can lead foreign genes to express efficiently and 90rercent of the expression products can secret to extracellular region; in pupae, the situation was similar to sarae, but expresses appeared to be heterogeneous. Expand
Analysis of N-Terminal Nucleotide Sequence of NDV Fusion Protein.
TLDR
Results show that the sequences of N-terminus are very similar, and cysteine residues and the potential glycosylation sites are relatively conserved, however, there are a few differences in the region of signal peptides. Expand
Transfer of shuttle vectors containing Bacillus thuringiensis toxin gene into wild-type B. cereus, B. brevis and B. subtilis by electroporation.
TLDR
Toxicity assays showed that the transformants gave a mortality of 90-100% against caterpillar of Heliothis assulta, indicating that the gene function was not changed by electroporation. Expand
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