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The family of glycogen phosphorylases: structure and function.
The functional properties of rabbit muscle phosphorylases are reviewed and then compared to properties of phosphoryLases from other tissues and organisms, and Evolutionary relationships among phosphory lases as afforded by comparative analysis of proteins and gene sequences are discussed. Expand
Molecular analysis of GPH1, the gene encoding glycogen phosphorylase in Saccharomyces cerevisiae.
GPH1 was found not to be an essential gene in yeast cells, and the low level of glycogen in exponential cells was not primarily maintained through regulating the phosphorylation state of a constitutive amount of phosphorylase. Expand
The structure of corepressor Dax-1 bound to its target nuclear receptor LRH-1
The Dax-1 protein is an enigmatic nuclear receptor that lacks an expected DNA binding domain, yet functions as a potent corepressor of nuclear receptors that binds to one of its targets, liver receptor homolog 1 (LRH-1). Expand
Chimeric Muscle and Brain Glycogen Phosphorylases Define Protein Domains Governing Isozyme-specific Responses to Allosteric Activation (*)
To identify the structural determinants involved, six enzyme forms, including four chimeric enzymes containing exchanges in amino acid residues 1-88, 89-499, and 500-842 (C terminus), were constructed from rabbit muscle and human brain phosphorylase cDNAs, expressed in Escherichia coli, and purified. Expand
Clathrin self-assembly is mediated by a tandemly repeated superhelix
The resulting model provides a three-dimensional framework for understanding clathrin heavy-chain self-assembly, light-chain binding and trimerization. Expand
Actin Binding by Hip1 (Huntingtin-interacting Protein 1) and Hip1R (Hip1-related Protein) Is Regulated by Clathrin Light Chain*
Clathrin is a negative regulator of Hip-actin interactions and these observations necessarily change models proposed for Hip protein function. Expand
Novel Binding Sites on Clathrin and Adaptors Regulate Distinct Aspects of Coat Assembly
The distinct sites for clathrin–adaptor interactions perform distinct functions, revealing new aspects to regulation of CCV formation. Expand
The Human Orphan Nuclear Receptor Tailless (TLX, NR2E1) Is Druggable
Nuclear receptors (NRs) are an important group of ligand-dependent transcriptional factors. Presently, no natural or synthetic ligand has been identified for a large group of orphan NRs. SmallExpand
A Protein Phosphorylation Switch at the Conserved Allosteric Site in GP
A phosphorylation-initiated mechanism of local protein refolding activates yeast glycogen phosphorylase (GP), and implicates glucose 6-phosphate, the allosteric inhibitor, in facilitating dephosphorylation by dislodging the buried covalent phosphate through binding competition. Expand
Structure-based Discovery of Antagonists of Nuclear Receptor LRH-1*
The data suggest that specific antagonists of LRH-1 could be used as specific molecular probes for elucidating the roles of the receptor in different types of malignancies. Expand