• Publications
  • Influence
[Pharmacokinetics and biotransformation of the analgesic flupirtine in humans].
TLDR
The pharmacokinetics of the analgesic flupirtine were examined in healthy volunteers after a single intravenous, peroral and rectal dose and two metabolites could be isolated from urine and their chemical structure determined. Expand
Interactions between redox partners in various cytochrome P450 systems: functional and structural aspects.
TLDR
Molecular modeling of the interactions between the redox components in various P450 mono-oxygenase systems is proposed on the basis of structural and mutagenesis information, together with experimental findings based on chemical modification of key residues likely to be associated with complementary binding sites on certain typical P450 isoforms and their respective redox partners. Expand
Assembly of non-natural electron transfer conduits in the cytochrome P450 system: a critical assessment and update of artificial redox constructs amenable to exploitation in biotechnological areas.
  • P. Hlavica
  • Chemistry, Medicine
  • Biotechnology advances
  • 1 March 2009
TLDR
The present review provides a critical update of the most significant breakthroughs in innovative manipulation of the redox machinery, giving an impulse to exploitation of P450s in fields such as the production of fine chemicals, drug processing, medicinal diagnostics and remediation of biotopes contaminated with harmful environmental pollutants. Expand
Allosteric phenomena in cytochrome P450-catalyzed monooxygenations.
TLDR
Dioxygen binding to cytochrome P450 displays nonhyperbolic kinetic profiles in the presence of certain substrates; the latter, together with redox proteins such as cy tochrome b5, can exert efficient control of the abortive breakdown of the oxyferrous intermediates formed. Expand
Functional interaction of cytochrome P450 with its redox partners: a critical assessment and update of the topology of predicted contact regions.
TLDR
The present review outlines the topological background of electron-transfer complex formation, showing that the progress in collaborative investigations, combining physical techniques with chemical-modification and immunolocalization studies as well as site-directed mutagenesis experiments, has increasingly enabled the substantiation of hypothetical work resulting from homology modelling of P450s. Expand
Biological oxidation of nitrogen in organic compounds and disposition of N-oxidized products.
  • P. Hlavica
  • Chemistry, Medicine
  • CRC critical reviews in biochemistry
  • 1982
TLDR
This work has shown that N-Oxidation of nitrogenous compounds has been shown to be of special biological interest: many N-oxidized aromatic amines have been recognized to be ultimate mutagens and carcinogens, and allergic disorders arise from interaction of such compounds with cellular macromolecules. Expand
MECHANISM OF N-OXIDE FORMATION BY HEPATIC MIXED FUNCTION OXIDASES
TLDR
Evidence is established for the existence of alternate metabolic routes of N-oxidation of N,N-dimethylaniline in rabbit liver microsomes that involve the participation of two types of cytochrome P-450 with differing sensitivity towards heat. Expand
The functional role of cytochrome b5 reincorporated into hepatic microsomal fractions.
TLDR
The inhibitory action of cytochrome b5 on the reduction of ferric cy tochrome P-450 is tentatively attributed to partial substitution of the former pigment for reductase in direct transport of the first electron to the monooxygenase. Expand
Oxidative biotransformation of fatty acids by cytochromes P450: predicted key structural elements orchestrating substrate specificity, regioselectivity and catalytic efficiency.
TLDR
Based on a general, CYP102A1-related construct, the majority of prospective key determinants participating in fatty acid recognition/binding were found to cluster near the distal heme face made up by the helical B', F, G and I tetrad as well as the B'-C interhelical loop and certain beta-sheet segments. Expand
Insect cytochromes P450: topology of structural elements predicted to govern catalytic versatility.
  • P. Hlavica
  • Chemistry, Medicine
  • Journal of inorganic biochemistry
  • 1 October 2011
TLDR
Detailed knowledge about structure–function relationships in insect P450s may offer an incentive to tailoring of novel insecticides and synergistic enzyme inhibitors to more efficiently control insect pests. Expand
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