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Dynamics of inner kinetochore assembly and maintenance in living cells
A wide range of cell cycle–specific assembly plasticity of the centromere that provides both stability through sustained binding of some components and flexibility through dynamic exchange of other components is revealed.
High- and low-mobility populations of HP1 in heterochromatin of mammalian cells.
Using fluorescence correlation spectroscopy and fluorescence recovery after photobleaching, data suggest that mobile as well as very slow populations of HP1 may function in concert to maintain a stable conformation of constitutive heterochromatin throughout the cell cycle.
Centrosomal localization of DNA damage checkpoint proteins
Results indicate that DNA damage checkpoint proteins may control the formation of γ‐tubulin and/or the kinetics of microtubule formation at the centrosomes, and thereby couple them to the DNA damage response.
Functional Complementation of Human Centromere Protein A (CENP-A) by Cse4p from Saccharomyces cerevisiae
Cse4p can structurally and functionally substitute for CENP-A, strongly suggesting that the basic features of centromeric chromatin are conserved between yeast and mammals.
Dynamics of component exchange at PML nuclear bodies
PML NB assembly dynamics were assessed by live cell imaging and mathematic modeling of its major component parts and it is shown that all six nuclear PML isoforms exhibit individual exchange rates at NBs and PML V as a scaffold subunit.
Cell cycle-dependent association of PML bodies with sites of active transcription in nuclei of mammalian cells.
The observations suggest that PML bodies may be recruited to nuclear sites of induced or up-regulated mRNA transcription where it may serve as a scaffold for factors involved in expression of specific genes.
Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq
Deep RNA sequencing the transcriptomes of five of the most commonly used laboratory strains of human fibroblasts during their transition into senescence provided an unprecedented comprehensive and deep view into the individual and common transcriptome and pathway changes.
Active-site probes of flavoproteins.
Flavins are very versatile coenzymes, functioning with considerable efficiency in a wide variety of enzymic reactions involving either two-electron or one-electron transfers, with both functions
Creb-Binding Protein (Cbp/P300) and RNA Polymerase II Colocalize in Transcriptionally Active Domains in the Nucleus
The results provide evidence for a dynamic spacial link between coactivators of transcription and the basal transcription machinery in discrete nuclear domains dependent upon the transcriptional activity of the cell.
Dynamic as well as stable protein interactions contribute to genome function and maintenance
An extension of the existing model for nuclear organization is proposed which now incorporates stable interactions in the nucleus which suggests that self-organization, self-assembly, and assisted assembly contribute to nuclear architecture and function.