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An Introduction to Reactive Oxygen Species Measurement of ROS in Cells
Reactive Oxygen Species (ROS) have long been known to be a component of the killing response of immune cells to microbial invasion. Recent evidence has shown that ROS play a key role as a messengerExpand
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Determination of Algal Cell Lipids Using Nile Red
Microalgal cultures have been used to produce natural products that have been derived into pharmaceuticals [1] and nutraceuticals [2] for a number of years. As a result of global climate change andExpand
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Protein-DNA interactions at the major and minor promoters of the divergently transcribed dhfr and rep3 genes during the Chinese hamster ovary cell cycle.
In mammals, two TATA-less bidirectional promoters regulate expression of the divergently transcribed dihydrofolate reductase (dhfr) and rep3 genes. In CHOC 400 cells, dhfr mRNA levels increase aboutExpand
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Fluorometric analysis of DNA in cell cultures.
A simple, reproducible fluorescent assay has been described which can be used confidently at microgram and submicrogram levels. The method is most suitable for cell cycle and cell growth analysisExpand
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Peroxiredoxin 3 Is a Redox-Dependent Target of Thiostrepton in Malignant Mesothelioma Cells
Thiostrepton (TS) is a thiazole antibiotic that inhibits expression of FOXM1, an oncogenic transcription factor required for cell cycle progression and resistance to oncogene-induced oxidativeExpand
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Mitochondrial‐targeted nitroxides disrupt mitochondrial architecture and inhibit expression of peroxiredoxin 3 and FOXM1 in malignant mesothelioma cells
Malignant mesothelioma (MM) is an intractable tumor of the peritoneal and pleural cavities primarily linked to exposure to asbestos. Recently, we described an interplay between mitochondrial‐derivedExpand
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Eukaryotic replication origins as promoters of bidirectional DNA synthesis.
Recent work in yeast shows that eukaryotic origins of DNA replication are multipartite regulatory elements resembling promoters of transcription. As for the regulation of transcription, accessoryExpand
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Measure Your Purity : Assessment of Nucleic Acid Purity via UV Absorbance
Molecular biologists commonly require assessment of nucleic acid sample purity through a spectrophotometric absorbance ratio of the sample at 260 nm compared to that at 280 nm. Impurities common toExpand
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Monitoring of Algal Growth Using their Intrinsic Properties
The increasing cost of fuel, as well as the impact of climate change, has dramatically increased the interest in alternative fuel sources. Biofuel production from algae is an area of keen on-goingExpand
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Protein-Induced Alterations in DNA Structure at the dhfr Origin of Replication
To identify the trans-acting factors that participate in initiation of DNA synthesis in mammalian cells, we have studied the interaction of nuclear proteins with the DNA sequences that encompass theExpand
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