P. Gourdon

Publications90
h-index22
Citations2,023
Highly Influential Citations134
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Light stimulates growth of proteorhodopsin-containing marine Flavobacteria
TLDR
The results show that the phototrophy conferred by proteorhodopsin can provide critical amounts of energy, not only for respiration and maintenance but also for active growth of marine bacterioplankton in their natural environment.
Crystal structure of a copper-transporting PIB-type ATPase
TLDR
The structure of a PIB-ATPase, a Legionella pneumophila CopA Cu+-ATpase, in a copper-free form, is presented, as determined by X-ray crystallography at 3.2 Å resolution, and indicates a three-stage copper transport pathway involving several conserved residues.
Crystal Structure of Na+, K+-ATPase in the Na+-Bound State
TLDR
The crystal structure of a Na+-bound Na+, K+-ATPase is presented, which reveals conformational changes associated with Na+ binding and the location of three bound sodium ions and the mechanism of sodium release in a key plasma membrane ion pump are revealed.
Crystal Structure of an Ammonia-Permeable Aquaporin
TLDR
By mutational studies, it is shown that the identified determinants in the extended selectivity filter region are sufficient to convert a strictly water-specific human aquaporin into an AtTIP2;1-like ammonia channel.
Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema
Copper-transporting P-type ATPases use a unique ion-release pathway
TLDR
It is shown by molecular dynamics simulations that extracellular water solvated the transmembrane (TM) domain, results indicative of a Cu+-release pathway, and a new LpCopA crystal structure determined at 2-Å resolution delineated the same passage, and site-directed-mutagenesis activity assays support a functional role for the conduit.
Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist.
Optimized in vitro and in vivo expression of proteorhodopsin: a seven-transmembrane proton pump.
Structure and mechanism of Zn2+-transporting P-type ATPases
TLDR
The findings suggest a mechanistic link between PIB-type Zn2+-ATPases and PIII-type H+-ATORases and at the same time show structural features of the extracellular release pathway that resemble PII-type ATPases such as the sarcoplasmic/endoplasmsic reticulum Ca2-atPase (SERCA) and Na+, K+- ATPase.
HiLiDe—Systematic Approach to Membrane Protein Crystallization in Lipid and Detergent
TLDR
An approach to achieve diffraction quality membrane protein crystals in high concentrations of lipid and detergent (HILIDE) is described, which is generally applicable for native and heterologously expressed membrane proteins, and it is compatible with any crystallization method.
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