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Isolation and Expression of Three Gibberellin 20-Oxidase cDNA Clones from Arabidopsis
Using degenerate oligonucleotide primers based on a pumpkin (Cucurbita maxima) gibberellin (GA) 20-oxidase sequence, six different fragments of dioxygenase genes were amplified by polymerase chainExpand
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The dwarf-1 (dt) Mutant of Zea mays blocks three steps in the gibberellin-biosynthetic pathway.
In plants, gibberellin (GA)-responding mutants have been used as tools to identify the genes that control specific steps in the GA-biosynthetic pathway. They have also been used to determine whichExpand
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Function and substrate specificity of the gibberellin 3beta-hydroxylase encoded by the Arabidopsis GA4 gene.
cDNA corresponding to the GA4 gene of Arabidopsis thaliana L. (Heynh. ) was expressed in Escherichia coli, from which cell lysates converted [14C]gibberellin (GA)9 and [14C]GA20 to radiolabeled GA4Expand
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The P450–1 gene of Gibberella fujikuroi encodes a multifunctional enzyme in gibberellin biosynthesis
Recent studies have shown that the genes of the gibberellin (GA) biosynthesis pathway in the fungus Gibberella fujikuroi are organized in a cluster of at least seven genes. P450–1 is one of fourExpand
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Internode length in Pisum
The influence of the Na and Le genes in peas on gibberellin (GA) levels and metabolism were examined by gas chromatographic-mass spectrometric analysis of extracts from a range of stem-lengthExpand
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Isolation and transcript analysis of gibberellin 20-oxidase genes in pea and bean in relation to fruit development
PCR was used with degenerate primers based on conserved amino acid sequences in gibberellin (GA) 20-oxidases to isolate cDNA clones for these enzymes from young seeds of pea (Pisum sativum) andExpand
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Cell wall alterations and localized accumulation of feruloyl-3-methoxytyramine in onion epidermis at sites of attempted penetration by Botrytis allii are associated with actin polarisation,
Summary Granular deposits of reaction material (RM) were formed in onion epidermal cells at sites of attempted penetration byBotrytis allii. Both RM and the adjacent cell wall fluoresced blue underExpand
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Deletions in the Gibberellin Biosynthesis Gene Cluster ofGibberella fujikuroi by Restriction Enzyme-Mediated Integration and Conventional Transformation-Mediated Mutagenesis
ABSTRACT We induced mutants of Gibberella fujikuroi deficient in gibberellin (GA) biosynthesis by transformation-mediated mutagenesis with the vector pAN7-1. We recovered 24 GA-defective mutants inExpand
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Feed-back regulation of gibberellin biosynthesis and gene expression in Pisum sativum L.
Treatment of tall and dwarf (3β-hydroxylase impaired) genotypes of pea (Pisum sativum L.) with the synthetic, highly active gibberellin (GA), 2,2-dimethyl GA4, reduced the shoot contents of C19-GAs,Expand
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