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Microscopic and Ultrastructural Studies of Necrotizing Hepatopancreatitis in Pacific White Shrimp (Penaeus vannamei) Cultured in Texas
Histologic and ultrastructural examination of shrimp from the four sites demonstrated two morphologically distinct, Gram-negative, double-enveloped, intracytoplasmic bacteria in necrotic hepatopancreatic epithelium and shrimp mortality correlated directly with the extent of infection by the small pleomorphic rod.
Molecular phylogeny and in situ detection of the etiologic agent of necrotizing hepatopancreatitis in shrimp
- J. K. Loy, F. Dewhirst, J. Templeton
- Biology, MedicineApplied and environmental microbiology
- 1 September 1996
Fluorescently labeled oligonucleotide DNA probes that bind to variable regions of 16S rRNA of the NHP bacterium were used to detect the bacterium in infected shrimp by in situ hybridization, providing direct visual evidence that the 16s rDNA that was amplified, cloned, and sequenced was derived from the intracellular bacterium that infects the hepatopancreas of farm-raised P. vannamei shrimp.
Red drum Sciaenops ocellatus mortalities associated with Streptococcus iniae infection.
Histopathological findings indicate that S. iniae infection in red drum produces a chronic disease with systemic involvement characterized by multiple necrotic foci, and 2 different ribotypes were involved in a single outbreak.
Streptococcus shiloi, the name for an agent causing septicemic infection in fish, is a junior synonym of Streptococcus iniae.
- A. Eldar, P. Frelier, L. Assenta, P. Varner, S. Lawhon, H. Bercovier
- BiologyInternational journal of systematic bacteriology
- 1 October 1995
DNA-DNA hybridization experiments revealed levels of homology of 77 to 100%.
Extrapallial abscesses associated with chronic bacterial infections in the intensively cultured juvenile Pacific oyster Crassostrea gigas
During a detailed survey of Pacific oyster Crassostrea gigas juvenile health at intensive rearing facilities, an episode of persistent morbidity and mortality occurred over an 8 wk period in 1997.…
Transmission of Necrotizing Hepatopancreatitis in Penaeus vannamei
The findings indicate that NHP is the result of a bacterial infection and that the small, pleomorphic, Gram-negative bacterium plays a major role in the pathogenesis of the disease.
Development of Specific Nested Oligonucleotide PCR Primers for the Streptococcus iniae 16S-23S Ribosomal DNA Intergenic Spacer
- B. Berridge, J. Fuller, J. D. de Azavedo, D. Low, H. Bercovier, P. Frelier
- BiologyJournal of Clinical Microbiology
- 1 September 1998
A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.
Otitis in feedlot cattle.
- P. Nation, P. Frelier, G. Gifford, B. Carnat
- MedicineThe Canadian veterinary journal = La revue…
- 1 July 1983
The significance of improved management, ventilation in particular, and the economic assessment of the significance of the diseases have provided incentives to reduce the effects of this disease in the cattle industry.
Prophylactic effect of monensin sodium against experimentally induced paratuberculosis in mice.
- G. Brumbaugh, P. Frelier, A. Roussel, T. D. Thomson
- Medicine, BiologyAmerican journal of veterinary research
- 1 April 1992
Infected mice given monensin had higher body weight and fewer hepatic granulomas than did mice not givenmonensin, and although hepaticgranulomas were fewer in these mice, they contained acid-fast organisms.