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Initial Reductive Reactions in Aerobic Microbial Metabolism of 2,4,6-Trinitrotoluene
The hydride-Meisenheimer complex of 2,4,6-trinitrotoluene was converted to a yellow metabolite, which by electrospray mass and nuclear magnetic resonance spectral analyses was established as the protonated dihydride-meisenheimercomplex of TNT (2H−-TNT). Expand
Catabolism of 3-Nitrophenol by Ralstonia eutropha JMP 134.
Ralstonia eutropha JMP 134 utilizes 3-nitrophenol as the sole source of nitrogen, carbon, and energy and enzyme-mediated transformation corresponds to the acid-catalyzed Bamberger rearrangement. Expand
Enzymatic formation, stability, and spontaneous reactions of 4-fluoromuconolactone, a metabolite of the bacterial degradation of 4-fluorobenzoate.
Enzymatic conversion of 4-fluorocatechol in the simultaneous presence of partially purified preparations of catechol 1,2-dioxygenase from Pseudomonas cepacia and muconate cycloisomerase fromExpand
Novel Type of Heme-Dependent Oxygenase Catalyzes Oxidative Cleavage of Rubber (Poly-cis-1,4-Isoprene)
An in vitro enzyme assay for oxidative rubber degradation was developed based on high-performance liquid chromatography analysis and spectroscopic detection of product carbonyl functions after derivatization with dinitrophenylhydrazone and confirmed the presence of heme. Expand
Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst.
Results demonstrate that an enzyme can be engineered to catalyze the transformation of substrates with structures widely divergent from those of its native substrate. Expand
3-(2-hydroxyphenyl)catechol as substrate for proximal meta ring cleavage in dibenzofuran degradation by Brevibacterium sp. strain DPO 1361.
The proximal mode of ring cleavage is definitely established by isolation and unequivocal structural characterization of a cyclization product of 2-hydroxy-6-(2-hydroxyphenyl)-6-oxo-2,4-hexadienoic acid, i.e., 3-(chroman-4-on-2-yl)pyruvate. Expand
Identification of a hydride-Meisenheimer complex as a metabolite of 2,4,6-trinitrotoluene by a Mycobacterium strain.
A bacterial strain, Mycobacterium sp. strain HL 4-NT-1, enriched with 4-nitrotoluene as its sole source of nitrogen, was able to metabolize 2,4,6-trinitrotoluene under aerobic conditions. The darkExpand
Inability of muconate cycloisomerases to cause dehalogenation during conversion of 2-chloro-cis,cis-muconate.
Both chloromuconolactones were found to be stable at physiological pH and must have evolved the capability to cleave the carbon-chlorine bond during their divergence from normal muconate cycloisomerases. Expand
A New 4-Nitrotoluene Degradation Pathway in aMycobacterium Strain
4-Hydroxylaminotoluene was converted to 6-amino-m-cresol by cell extracts and thus could be confirmed as the initial metabolite in the degradative pathway and was identified as the enzymatic equivalent to the acid-catalyzed Bamberger rearrangement. Expand
Converging catabolism of 2,4,6-trinitrophenol (picric acid) and 2,4-dinitrophenol by Nocardioides simplex FJ2-1A
A convergent catabolic pathway for picrate and 2,4-dinitrophenol with thehydride σ-complex of 2,6-trinitropenol as the common intermediate has been demonstrated and an enzymatic activity has been identified catalyzing the release of stoichometric amounts of 1 mol nitrite from 1 mol of the dihydrideπ-complexof picrate. Expand