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Purification of the yeast PHR1 photolyase from an Escherichia coli overproducing strain and characterization of the intrinsic chromophores of the enzyme.
We have placed the PHR1 gene of Saccharomyces cerevisiae under the transcriptional and translational control of the tac expression cartridge. Under inducing conditions Escherichia coli cellsExpand
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Photophysical and photochemical characterization of a photosensitizing drug: a combined steady state photolysis and laser flash photolysis study on carprofen.
Carprofen (1a) is a photosensitizing nonsteroidal anti-inflammatory drug. It undergoes photodehalogenation from its triplet excited state. The resulting aryl radical (II) is able to abstract hydrogenExpand
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Photochemical properties of Escherichia coli DNA photolyase: a flash photolysis study.
Escherichia coli DNA photolyase contains a stable flavin neutral blue radical that is involved in photosensitized repair of pyrimidine dimers in DNA. We have investigated the effect of illuminationExpand
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Pathways of electron transfer in Escherichia coli DNA photolyase: Trp306 to FADH.
We describe the results of a series of theoretical calculations of electron transfer pathways between Trp306 and *FADH. in the Escherichia coli DNA photolyase molecule, using the method ofExpand
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The active form of Escherichia coli DNA photolyase contains a fully reduced flavin and not a flavin radical, both in vivo and in vitro.
Escherichia coli DNA photolyase is a flavoprotein that when purified is blue in color and contains a stable neutral radical FAD (E-FADH). In the presence of a suitable electron donor (i.e., thiols,Expand
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Photochemical properties of Escherichia coli DNA photolyase: selective photodecomposition of the second chromophore.
Escherichia coli DNA photolyase contains a stable flavin radical and a second chromophore (SC) of unknown structure. The effects of flash (both conventional and laser) excitation of either theExpand
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Energy transfer (deazaflavin-->FADH2) and electron transfer (FADH2-->T <> T) kinetics in Anacystis nidulans photolyase.
DNA photolyases catalyze the photocycloreversion of cyclobutane pyrimidine dimers. The enzyme from the cyanobacterium Anacystis nidulans contains two chromophores, 1,5-dihydroflavin adenineExpand
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The Photochemistry of Flavins
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A LASER FLASH PHOTOLYSIS STUDY OF THE NATURE OF FLAVIN MONONUCLEOTIDE TRIPLET STATES AND THE REACTIONS OF THE NEUTRAL FORM WITH AMINO ACIDS
Abstract— Primary photochemical processes in aqueous solution have been characterised for FMN. The influence of pH on these processes is attributed to protonation of the neutral triplet and not toExpand
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