Phosphorylation of human plasma alpha2-Heremans-Schmid glycoprotein (human fetuin) in vivo.
- Å. Haglund, B. Ek, P. Ek
- Biology, ChemistryBiochemical Journal
- 15 July 2001
This finding, for the first time, that circulating human plasma fetuin is partly phosphorylated, implies that the effects of phosphorylation alpha2-HS glycoprotein on insulin signal transduction seen in different cell systems could be relevant to its physiological function in vivo.
Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase.
- P. Ek, G. Pettersson, B. Ek, Feng Gong, Jin-ping Li, O. Zetterqvist
- BiologyEuropean Journal of Biochemistry
- 1 October 2002
The results provide a new tool for studying eukaryotic histidine phosphorylation/dephosphorylation and indicated that the human phosphohistidine phosphatase mRNA was present preferentially in heart and skeletal muscle.
Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer
- Gholamreza Tavoosidana, G. Ronquist, M. Kamali-Moghaddam
- Biology, MedicineProceedings of the National Academy of Sciences
- 9 May 2011
An extremely sensitive and specific assay for detection of complex target structures such as microvesicles in which the target is first captured via an immobilized antibody and subsequently detected by using four other antibodies with attached DNA strands is developed.
Adenosine‐5′‐carboxylic Acid Peptidyl Derivatives as Inhibitors of Protein Kinases.
- M. Loog, A. Uri, G. Raidaru, J. Jaerv, P. Ek
- Chemistry
- 5 October 1999
Characterization of protein kinase C‐mediated phosphorylation of the short cytoplasmic domain isoform of C‐CAM
- M. Edlund, K. Wikström, R. Toomik, P. Ek, B. Öbrink
- Biology, ChemistryFEBS Letters
- 20 March 1998
Peptide phosphorylation by calcium-dependent protein kinase from maize seedlings.
- M. Loog, R. Toomik, K. Sak, G. Muszyńska, J. Järv, P. Ek
- BiologyEuropean Journal of Biochemistry
- 2000
Ca2+-dependent protein kinase (CDPK-1) was purified from maize seedlings, and its substrate specificity studied using a set of synthetic peptides derived from the phosphorylatable sequence…
Phosphorylation of plant proteins and the identification of protein-tyrosine kinase activity in maize seedlings.
- J. Trojánek, P. Ek, J. Scoble, G. Muszyńska, L. Engström
- Biology, ChemistryEuropean Journal of Biochemistry
- 1996
Chemical, immunological and enzymatic data indicated the presence of tyrosine kinase activity and also phosphotyrosine in proteins of maize seedlings and the plant enzyme(s) is reminiscent known mammalian cytosolic tyrosines kinase(s).
Kinetic study of the inhibition of CK2 by heparin fragments of different length.
- F. O'Farrell, M. Loog, I. Janson, P. Ek
- Biology, ChemistryBiochimica et Biophysica Acta
- 17 August 1999
A potent and highly selective peptide substrate for protein kinase C assay.
The favourable kinetic parameters combined with the selectivity should make the KRAKRKTAKKR peptide useful as a substrate for protein kinase C in the assays of both purified enzyme and in crude cell extracts.
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