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Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis.
TLDR
The cell-wall phenotype of irx7, irx8 and irx9, three previously described irregular xylem (irx) mutants, and irX14 and parvus-3 are shown to be secondary cell- wall mutants, which have mutations in genes encoding putative glycosyltransferases and exhibited large decreases in xylan. Expand
Analysis of Detergent-Resistant Membranes in Arabidopsis. Evidence for Plasma Membrane Lipid Rafts1
TLDR
The results indicate that the preparation of DRMs can yield a very specific set of membrane proteins and suggest that the PM contains phytosterol and sphingolipid-rich lipid domains with a specialized protein composition. Expand
Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components
TLDR
It is demonstrated that copper is needed for GH61 maximal activity and that the formation of cellodextrin and oxidized cellodesxtrin products by GH61 is enhanced in the presence of small molecule redox-active cofactors such as ascorbate and gallate. Expand
Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis.
TLDR
Characteristics are similar to those of irx9 and irx14, mutants that are believed to be defective in xylan chain elongation, and suggests that IRX10 and IRx10-L also play a role in elongation of the xylan backbone. Expand
Identification of Glycosylphosphatidylinositol-Anchored Proteins in Arabidopsis. A Proteomic and Genomic Analysis1
TLDR
This work presents the first, to the authors' knowledge, large-scale proteomic identification of plant GAPs, and confirms the existence of a large number of phospholipase C-sensitive Arabidopsis proteins. Expand
Glycosylphosphatidylinositol Lipid Anchoring of Plant Proteins. Sensitive Prediction from Sequence- and Genome-Wide Studies for Arabidopsis and Rice1
TLDR
The “big-Π plant” program for prediction of compatibility of query protein C-termini with the plant GPI lipid anchor motif requirements is developed and can be applied as unsupervised genome annotation and target selection tool. Expand
COBRA, an Arabidopsis Extracellular Glycosyl-Phosphatidyl Inositol-Anchored Protein, Specifically Controls Highly Anisotropic Expansion through Its Involvement in Cellulose Microfibril Orientationw⃞
TLDR
Characterization of a null allele, cob-4, establishes the key role of COB in controlling anisotropic expansion in most developing organs and suggests that COB, through its involvement in cellulose microfibril orientation, is an essential factor in highly anisotrop expansion during plant morphogenesis. Expand
VIP21, a 21-kD membrane protein is an integral component of trans-Golgi- network-derived transport vesicles
TLDR
It is proposed that VIP21, a cDNA encoding one protein of this complex, VIP21 (Vesicular Integral-membrane Protein of 21 kD), is a component of the molecular machinery of vesicular transport. Expand
Mapping the Arabidopsis organelle proteome.
TLDR
The localization of organelle proteins by the isotope tagging technique in conjunction with isotope tags for relative and absolute quantitation and 2D liquid chromatography for the simultaneous assignment of proteins to multiple subcellular compartments has enabled protein steady-state distributions to be determined. Expand
Absence of branches from xylan in Arabidopsis gux mutants reveals potential for simplification of lignocellulosic biomass
TLDR
The xylan in these plants shows improved extractability from the cell wall, is composed of a single monosaccharide, and requires fewer enzymes for complete hydrolysis, demonstrating the potential for manipulating and simplifying the structure of xylan to improve the properties of lignocellulose for bioenergy and other uses. Expand
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