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Development of a Rapid and Sensitive Test for Identification of Major Pathogens in Bovine Mastitis by PCR
TLDR
The two PCR tests avoid cumbersome and lengthy cultivation steps, can be performed within hours, and are sensitive, specific, and reliable for the direct detection in milk of the six most prevalent bacteria causing bovine mastitis.
Masitinib is safe and effective for the treatment of canine mast cell tumors.
TLDR
Masitinib is safe and effective at delaying tumor progression in dogs presenting with recurrent or nonresectable grade II or III nonmetastatic MCT.
Long‐chain omega‐3 fatty acids regulate bovine whole‐body protein metabolism by promoting muscle insulin signalling to the Akt–mTOR–S6K1 pathway and insulin sensitivity
TLDR
It is concluded that chronic feeding of menhaden oil provides a novel nutritional mean to enhance insulin‐sensitive aspects of protein metabolism.
Intestinal disappearance and mesenteric and portal appearance of amino acids in dairy cows fed ruminally protected methionine.
TLDR
The addition of ruminally protected Met to the diet increased the duodenal flux of Met leading to a higher apparent digestibility of Met in the small intestine, leading to higher concentrations of urea-N and glucose in arterial plasma.
The route of absorbed nitrogen into milk protein
A database reviewing the metabolism of nitrogen (N) compounds from absorption to milk has been compiled from 14 published and unpublished studies (33 treatments) that measured the net flux of N
Effect of level of metabolizable protein on splanchnic flux of amino acids in lactating dairy cows.
TLDR
With increasing MP, the ratio of milk output to postliver supply of BCAA, Thr, and Lys decreased linearly, indicating oxidation of these AA in the peripheral tissues, and the efficiency of transfer of absorbed EAA into milk protein decreases markedly as protein supply increases.
A new PCR-based seroneutralization assay in cell culture for diagnosis of hepatitis E
TLDR
A new method for the serological diagnosis of hepatitis E virus (HEV) infection based on neutralization of the virus in cell culture was developed and is proposed as a confirmatory test for the available enzyme-linked immunosorbent assay (ELISA), which is now recognized as giving many false-positive reactions.
Evaluation of Different Cytomegalovirus (CMV) DNA PCR Protocols for Analysis of Dried Blood Spots from Consecutive Cases of Neonates with Congenital CMV Infections
ABSTRACT Two protocols for the extraction of cytomegalovirus (CMV) DNA and two methods for the amplification of CMV DNA in dried blood spots were evaluated for the retrospective diagnosis of
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