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The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.
A theoretical analysis of the oxidation of 3,5,3',5'-tetramethylbenzidine is presented, including a determination of the extinction coefficients and equilibrium constant for the nonradical species. Expand
Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli.
The identified genetic locus, soxR (superoxide response), that positively regulates 9 of these proteins during superoxide stress may operate as an inducible defense against xenobiotics in general. Expand
Genetic Variations in Human Glutathione Transferase Enzymes: Significance for Pharmacology and Toxicology
  • P. D. Josephy
  • Biology, Medicine
  • Human genomics and proteomics : HGP
  • 13 June 2010
This review presents a discussion of the biochemistry of GSTs, the sources—both genetic and environmental—of interindividual variation in GST activities, and their implications for pharmaco- and toxicogenetics; particular attention is paid to the Theta class GSTs. Expand
Comparison of the AmesSalmonellaAssay and Mutatox Genotoxicity Assay for Assessing the Mutagenicity of Polycyclic Aromatic Compounds in Porewater from Athabasca Oil Sands Mature Fine Tailings
The oil sands in the Athabasca region of northeastern Alberta, Canada, represent a significant hydrocarbon resource that is currently exploited by mining, followed by separation of bitumen from sandExpand
A comparison of the anticarcinogenic properties of four red wine polyphenols.
It is shown recently that trans-resveratrol is absorbed much more efficiently than (+)-catechin and quercetin in humans after oral consumption and may be the most effective anticancer polyphenol present in red wine as consumed by healthy human subjects. Expand
Selection and characterization of human cytochrome P450 1A2 mutants with altered catalytic properties.
Systems for generation of random libraries of human P450 1A2 with the putative substrate recognition sequences mutated and an Escherichia coli genotoxity assay involving reversion to lac prototrophy as a response to activation of the heterocyclic amine 2-amino-3-5-dimethylimidazo[4,5-f]quinoline (MeIQ). Expand
Functional characterization of four allelic variants of human cytochrome P450 1A2.
Human cytochrome P450 1A2 catalyzes important reactions in xenobiotic metabolism, including the N-hydroxylation of carcinogenic aromatic amines and O-deethylation of phenacetin, and these variants had subtly different catalytic activities compared to the wild-type enzyme. Expand
The Escherichia coli lacZ reversion mutagenicity assay.
The Escherichia coli lacZ reversion assay, based on the set of episomal lacZ alleles engineered by Miller et al., provides an attractive system for studies of mutagenesis and mutational specificity.Expand
Single-nucleotide polymorphic variants of human glutathione transferase T1-1 differ in stability and functional properties.
The results show that SNP variants can give rise to GSTT1-1 proteins with significantly altered properties. Expand
Bioactivation of aromatic amines by recombinant human cytochrome P4501A2 expressed in Ames tester strain bacteria: a substitute for activation by mammalian tissue preparations.
It is shown that the requirement for S9 can be obviated by the engineered expression of enzymes of bioactivation within the bacterial cell, and an Ames tester strain is constructed, expressing both aromatic amine N-acetyltransferase and human cytochrome P4501A2, which detects aromaticAmine mutagenicity in the absence of S9. Expand