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Distribution of nitrogen fixation and nitrogenase-like sequences amongst microbial genomes
- P. D. Dos Santos, Z. Fang, Steven Mason, J. Setubal, R. Dixon
- Biology, Medicine
- BMC Genomics
- 3 May 2012
A new criterion for computational prediction of nitrogen fixation is proposed: the presence of a minimum set of six genes coding for structural and biosynthetic components, namely NifHDK and NifENB. Expand
NifS-mediated assembly of [4Fe-4S] clusters in the N- and C-terminal domains of the NifU scaffold protein.
- Archer D. Smith, G. Jameson, +7 authors Michael K. Johnson
- Biology, Medicine
- 4 October 2005
Both scaffolding domains of NifU are shown to be competent for in vitro maturation of nitrogenase component proteins, as evidenced by rapid transfer of [4Fe-4S](2+) clusters preassembled on either the N- or C-terminal domains to the apo nitrogenase Fe protein. Expand
Genome Sequence of Azotobacter vinelandii, an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes
The complete genome sequence of A. vinelandii DJ is reported, which identified the chromosomal locations of the genes coding for the three known oxygen-sensitive nitrogenases, as well as genes codes for other oxygen- sensitive enzymes, such as carbon monoxide dehydrogenase and formate dehydrogen enzyme. Expand
A Proposed Role for the Azotobacter vinelandii NfuA Protein as an Intermediate Iron-Sulfur Cluster Carrier*
- Sibali Bandyopadhyay, S. Naik, +4 authors P. D. Dos Santos
- Chemistry, Medicine
- Journal of Biological Chemistry
- 16 May 2008
Spectroscopic and analytical studies indicate that one [4Fe-4S] cluster can be assembled in vitro within a dimeric form of NfuA, which is competent for rapid in vitro activation of apo-aconitase. Expand
In vivo iron–sulfur cluster formation
- E. C. Raulfs, I. P. O'Carroll, P. D. Dos Santos, M. Unciuleac, D. Dean
- Chemistry, Medicine
- Proceedings of the National Academy of Sciences
- 24 June 2008
It is shown that production of the intact Azotobacter vinelandii [Fe-S] cluster biosynthetic machinery at levels exceeding the amount required for cellular maturation of [ Fe-S], and substitution of the IscU Asp39 residue by Ala results in the in vivo trapping of a stoichiometric, noncovalent, nondissociating Isc U–IscS complex that contains an oxygen-resistant [Fe -S] species. Expand
Transcriptional Profiling of Nitrogen Fixation in Azotobacter vinelandii
Genome-wide transcription profiling of A. vinelandii cultured under nitrogen-fixing conditions under various metal amendments revealed the discrete complement of genes associated with each nitrogenase system and the extent of cross talk between the systems, which underscored significant differences between Mo-dependent and Mo-independent diazotrophic growth. Expand
Characterization of the N-acetyl-α-D-glucosaminyl l-malate synthase and deacetylase functions for bacillithiol biosynthesis in Bacillus anthracis .
The first two enzymes for the BSH biosynthetic pathway in B. anthracis are characterized, and the crystal structure of the BaBshA-UDP-malate ternary complex, determined in this work at 3.3 Å resolution, identifies several active-site interactions important for the specific recognition of l- malate, but not other α-hydroxy acids, as the acceptor substrate. Expand
Molecular biology and genetic engineering in nitrogen fixation.
- P. D. Dos Santos
- Methods in molecular biology
This chapter includes protocols and strategies in the molecular biology and genetic engineering of A. vinelandii that have been used as valuable tools for advancing studies on the biosynthesis, mechanism, and regulation of nitrogen fixation. Expand
Mammalian Transforming Growth Factor β1 Activated after Ingestion by Anopheles stephensi Modulates Mosquito Immunity
Observations indicate that, through conserved immunological cross talk, mammalian and mosquito immune systems interface with each other to influence the cycle of Plasmodium development. Expand
Shared-intermediates in the biosynthesis of thio-cofactors: Mechanism and functions of cysteine desulfurases and sulfur acceptors.
Understanding the roles of promiscuous vs. dedicated cysteine desulfurases and their partnership with shared-intermediates in the biosynthesis of thio-cofactors will help to map sulfur transfer events across interconnected pathways and to provide insight into the hierarchy of sulfur incorporation into biomolecules. Expand