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Metabolite profiling for plant functional genomics
TLDR
The use of metabolite profiling is described as a new tool for a comparative display of gene function and has the potential not only to provide deeper insight into complex regulatory processes but also to determine phenotype directly. Expand
Vitamin E Protects against Photoinhibition and Photooxidative Stress in Arabidopsis thaliana
TLDR
It is concluded that vitamin E fulfills at least two different functions in chloroplasts at the two major sites of singlet oxygen production: preserving PSII from photoinactivation and protecting membrane lipids from photooxidation. Expand
Isolation of an Arabidopsis mutant lacking vitamin E and identification of a cyclase essential for all tocopherol biosynthesis
TLDR
Growth of the vte1 mutant, chlorophyll content, and photosynthetic quantum yield were similar to wild type under optimal growth conditions, suggesting that other antioxidants can compensate for the loss of tocopherol. Expand
Galactolipids rule in seed plants.
TLDR
Galactolipids are found in X-ray structures of photosynthetic complexes, suggesting a direct role in photosynthesis and can substitute for phosphate deprivation, as suggested by increases in the galactolIPid:phospholipid ratio after phosphate deprivation. Expand
Disruption of the Two Digalactosyldiacylglycerol Synthase Genes DGD1 and DGD2 in Arabidopsis Reveals the Existence of an Additional Enzyme of Galactolipid Synthesis Article, publication date, and
TLDR
The role of DGD2 for galactolipid synthesis was studied by isolating Arabidopsis T-DNA insertional mutant alleles and generating the double mutant line dgd1 dgd2, with results indicating that the residual amount of D GDG in dgd 1 is crucial for normal plant development. Expand
GMD@CSB.DB: the Golm Metabolome Database
TLDR
GD, The Golm Metabolome Database is presented, an open access metabolome database, which provides public access to custom mass spectral libraries, metabolite profiling experiments as well as additional information and tools, e.g. with regard to methods, spectral information or compounds. Expand
Tocopherol Cyclase (VTE1) Localization and Vitamin E Accumulation in Chloroplast Plastoglobule Lipoprotein Particles*
TLDR
Comparison of chloroplast membrane fractions shows that plastoglobules are a site of vitamin E accumulation in chloroplasts, and it is proposed that plastsoglobule are metabolically active, taking part in tocopherol synthesis and likely other pathways. Expand
Phospholipase C5 (NPC5) is involved in galactolipid accumulation during phosphate limitation in leaves of Arabidopsis.
TLDR
Gene expression and lipid analyses revealed that the MYB transcription factor PHR1 that has been previously shown to regulate phosphate responses is not a major factor controlling membrane lipid changes, and soluble NPC5 associates with membranes where it contributes to the conversion of phospholipids to diacylglycerol, the substrate for galactolipid synthesis. Expand
Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature[W]
TLDR
It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins, which is evolutionary conserved in plants and cyanobacteria. Expand
Alterations in Tocopherol Cyclase Activity in Transgenic and Mutant Plants of Arabidopsis Affect Tocopherol Content, Tocopherol Composition, and Oxidative Stress1
TLDR
expression studies demonstrated that indeed VTE1 is a major limiting factor of tocopherol synthesis in leaves, and the simultaneous loss of toCopherol and glutathione results in moderate oxidative stress that affects the stability and the efficiency of the photosynthetic apparatus. Expand
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