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Selective enzyme purification by affinity chromatography.
The general principles and potential applications of "affinity chromatography," a protein purification technique that is indispensable to modern biological research, are explained. Expand
A simplified method for cyanogen bromide activation of agarose for affinity chromatography.
Abstract Agarose can be activated by adding cyanogen bromide, dissolved in acetonitrile, to beads suspended in a solution of sodium carbonate. The necessity for manual titration and the use of a pHExpand
Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.
A spectrophotometric assay for staphylococcal nuclease is described, based on the increase in absorbance at 260 mµ which accompanies deoxyribonucleic acid and RNA hydrolysis, to suggest that the differential activity toward DNA and RNA results at least in part from differences in the affinity toward the constituent bases of these nucleic acids. Expand
Mitogen-activated Protein Kinase Kinase Inhibition Does Not Block the Stimulation of Glucose Utilization by Insulin (*)
The results indicate that stimulation of the MAPK pathway by insulin is not required for many of the metabolic activities of the hormone in cultured fat and muscle cells. Expand
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
  • P. Cuatrecasas
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • 25 June 1970
It is demonstrated that successful application of affinity chromatography in many cases will critically depend on placing the ligand at a considerable distance from the matrix backbone. Expand
Cellular receptor for 125I-labeled tumor necrosis factor: specific binding, affinity labeling, and relationship to sensitivity.
The presence of the binding site appears to be necessary but not sufficient to explain the sensitivity of cells to the cytotoxic action of TNF, and two human tumorigenic cell lines were not sensitive and failed to demonstrate specific binding. Expand
Protein kinase C phosphorylates topoisomerase II: topoisomerase activation and its possible role in phorbol ester-induced differentiation of HL-60 cells.
DNA topoisomersase II from Drosophila was phosphorylated effectively by protein kinase C and resulted in appreciable activation of the topoisomerase, suggesting that it may represent a possible target for the regulation of nuclear events by protein Kinase C. Expand
Monoclonal antibodies to receptors for insulin and somatomedin-C.
Three monoclonal antibodies were prepared by fusing FO myeloma cells with spleen cells from a mouse immunized with a partially purified preparation of insulin receptors from human placenta to immunoprecipitate insulin and somatomedin-C receptors from solubilized human lymphoid cells and human Placenta membranes that had been 125I-labeled with lactoperoxidase. Expand
Interaction of Vibrio cholerae enterotoxin with cell membranes.
Immunohistochemical localization of enkephalin in rat brain and spinal cord
The results of the histochemical staining show that certain structures which positively stain for enkephalin closely correspond to the distribution of opiate receptors in the brain and thus support the concept that the endogenous opiate peptides are involved in the perception of pain and analgesia. Expand