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Construction of three new Gateway® expression plasmids for Trypanosoma cruzi
Three expression plasmids for Trypanosoma cruzi adapted to the Gateway® recombination cloning system were presented, designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag) and the inducible pTcINDEX. Expand
Overexpression of Cytoplasmic TcSIR2RP1 and Mitochondrial TcSIR2RP3 Impacts on Trypanosoma cruzi Growth and Cell Invasion
The data suggest that sirtuin activity is important for the proliferation of T. cruzi replicative forms, for the host cell-parasite interplay, and for differentiation among life-cycle stages; but each one performs different roles in most of these processes. Expand
One- and two-hybrid analysis of the interactions between components of the Trypanosoma cruzi spliced leader RNA gene promoter binding complex.
  • P. Cribb, E. Serra
  • Biology, Medicine
  • International journal for parasitology
  • 1 April 2009
The results suggest that the SL RNA gene promoter binding complex has two "cores" which contact the promoter DNA, trypanosomal small nuclear RNA activating protein complex (tSNAPc), which has strong interactions between its subunits and a more labile TBP-TFIIA sub-complex. Expand
Glycosomal bromodomain factor 1 from Trypanosoma cruzi enhances trypomastigote cell infection and intracellular amastigote growth.
The first characterization of Trypanosoma cruzi bromodomain factor 1 (TcBDF1) is reported, which localizes in the glycosomes directed by a PTS2 (peroxisome-targeting signal 2) sequence and is developmentally regulated. Expand
One-tube nested polymerase chain reaction for detection of Chlamydia trachomatis.
A one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids, and is robust enough to be used for clinical diagnosis. Expand
Trypanosoma cruzi High Mobility Group B (TcHMGB) can act as an inflammatory mediator on mammalian cells
Preliminary results suggest that TcHMGB can act as an exogenous immune mediator that may be important for both the control of parasite replication as the pathogenesis of Chagas disease and can be envisioned as a pathogen associated molecular pattern (PAMP) partially overlapping in function with the host DAMPs. Expand
Trypanosoma cruzi bromodomain factor 2 (BDF2) binds to acetylated histones and is accumulated after UV irradiation.
It is shown that one of those, TcBDF2, is expressed in discrete regions inside the nucleus of all the parasite life cycle stages and binds H4 and H2A purified histones from T. cruzi. Expand
Spliced leader RNA gene promoter sequence heterogeneity in CL-Brener Trypanosoma cruzi reference strain.
The results presented herein show that sequence heterogeneity in SL RNA gene promoter not only exists between T. cruzi strains but also within CL-Brener strain, and could be considered a molecular trace of a hybrid origin of the SLRNA gene and a new evidence for the presence of sequences of T. Cruzi I origin into a T.cruzi II strain. Expand
Overexpression of bromodomain factor 3 in Trypanosoma cruzi (TcBDF3) affects differentiation of the parasite and protects it against bromodomain inhibitors
It is shown that parasites overexpressing TcBDF3 exhibit altered differentiation patterns and are less susceptible to treatment with bromodomain inhibitors, and it is demonstrated that recombinant Tc BDF3 is able to bind to these inhibitors in vitro in a concentration‐dependant manner. Expand
Trypanosoma cruzi Bromodomain Factor 3 Binds Acetylated α-Tubulin and Concentrates in the Flagellum during Metacyclogenesis
ABSTRACT Bromodomains are highly conserved acetyl-lysine binding domains found mainly in proteins associated with chromatin and nuclear acetyltransferases. The Trypanosoma cruzi genome encodes atExpand