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Fungal multienzyme production on industrial by-products of the citrus-processing industry.
Production of multienzyme preparations containing pectinolytic, cellulolytic and xylanolytic enzymes by the mesophilic fungi Aspergillus niger BTL, Fusarium oxysporum F3, Neurospora crassa DSM 1129 and Penicillium decumbens under solid-state fermentation on dry orange peels was enhanced by optimization of initial pH of the culture medium and initial moisture level.
Microbial production, characterization and applications of feruloyl esterases
FAEs act synergistically with xylanases to hydrolyze ester-linked ferulic and diferulic acid from cell wall material and therefore play a major role in the degradation of plant biomass.
Hydrolysis and fermentation of brewer's spent grain by Neurospora crassa.
- Charilaos Xiros, E. Topakas, P. Katapodis, P. Christakopoulos
- Chemistry, MedicineBioresource technology
- 1 September 2008
Chromogenic (fluorogenic) 4-methylumbelliferyl substrates were used to characterize the major activities of the multienzyme component, after the separation by isoelectric focusing (IEF) electrophoresis.
Enhanced ethanol production from brewer's spent grain by a Fusarium oxysporum consolidated system
The enzymatic profile of the extracellular extract from F. oxysporum submerged cultures using BG and corn cob as the carbon source was proved efficient for a successful hydrolysis of BG, indicating that BG Hydrolysis is the bottleneck of the bioconversion process.
Performance of an intermittent agitation rotating drum type bioreactor for solid-state fermentation of wheat straw.
- E. Kalogeris, F. Iniotaki, E. Topakas, P. Christakopoulos, D. Kekos, B. Macris
- Biology, MedicineBioresource technology
- 1 February 2003
The suitability of the apparatus for the effective control of important operating variables affecting growth of microbes in solid-state cultivation was determined and the correlation between microorganism growth and production of enzymes was efficiently described by the Le Duy kinetic model.
Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis
Abstract A feruloyl esterase (StFaeC) produced by Sporotrichum thermophile was purified to homogeneity. The native StFaeC was homodimer with a subunit of M r 23,000 and pI 3.1. The enzyme activity…
An alternative approach to the bioconversion of sweet sorghum carbohydrates to ethanol
The ethanol fermentation of juice and press cake, resulting from the squeezing of sweet sorghum stalks at high pressure, was investigated. The juice was fermented by Saccharomyces cerevisiae and…
Purification and characterization of a type B feruloyl esterase (StFAE-A) from the thermophilic fungus Sporotrichum thermophile
- E. Topakas, H. Stamatis, P. Biely, P. Christakopoulos
- Chemistry, MedicineApplied Microbiology and Biotechnology
- 1 February 2004
The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose and it hydrolyzed 4-nitrophenyl 5-O-trans-feruloyl-α-l-arabinofuranoside 2-fold more efficiently than NPh-2-Fe-Araf.
Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes
Elevated levels of endoglucanase and β-glucosidase activities were produced simultaneously by optimization of growth factors by the thermophilic fungus Thermoascus aurantiacus, and both enzymes exhibited significant thermostability.
Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Thermomyces lanuginosus ATCC 46882.
An endoxylanase from T. lanuginosus ATCC 46882 was purified to homogeneity by DEAE-Sepharose and Bio-Gel P-30 column chromatographies and suggested that it belongs to family 11.2.